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Construction and Characterization of anagr Deletion Mutant of Staphylococcus epidermidis

机译：表皮葡萄球菌anagr缺失突变体的构建与表征

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The physiological significance of the accessory gene regulator (agr) system of Staphylococcus epidermidis was investigated by construction of an agr deletion mutant via allelic replacement with a spectinomycin resistance cassette. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that the protein pattern was strongly altered in the mutant; the amounts of most surface proteins were higher, whereas the amounts of most exoproteins were lower. The agrsystem of S. epidermidis thus appears to have an important impact on growth phase-dependent protein synthesis as has been shown for Staphylococcus aureus. The activity of the exoenzymes lipase and protease, assumed to be involved in staphylococcal pathogenicity, was investigated by agar diffusion assays and SDS-PAGE activity staining. A general reduction of these enzyme activities in the agr mutant was found. The difference in overall lipase activity was small, but zymographic analysis suggested a clear defect in lipase processing in the agr mutant.
机译：表皮葡萄球菌的辅助基因调节剂（agr）系统的生理学意义是通过用壮观霉素抗性盒等位基因置换构建一个agr缺失突变体来研究的。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析表明该突变体中的蛋白质模式发生了强烈变化。大多数表面蛋白的含量较高，而大多数外蛋白的含量较低。因此，表皮葡萄球菌的农业系统似乎对金黄色葡萄球菌已显示出对生长阶段依赖性蛋白质合成具有重要影响。通过琼脂扩散分析和SDS-PAGE活性染色研究了假定与葡萄球菌致病性有关的外切酶脂肪酶和蛋白酶的活性。发现在agr突变体中这些酶活性普遍降低。总体脂肪酶活性的差异很小，但酶谱分析表明在agr突变体中脂肪酶加工过程中存在明显缺陷。

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