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Effect of estrogens on bacterial adherence to HeLa cells.

机译:雌激素对细菌对HeLa细胞粘附的影响。

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Incubating confluent cell culture HeLa cells for 18 h with increasing concentrations of estrogens progressively enhanced the subsequent attachment of a variety of radiolabeled bacteria to the HeLa cells. This effect was not caused by other hormones and was not produced by 1-h incubations of HeLa cells or bacteria with hormones. Estrogens did not similarly affect two other receptor cell lines studied. The addition of metabolic inhibitors showed that this effect of estrogens on HeLa cells was energy dependent and involved protein synthesis. Concurrent incubation of the HeLa cells and estrogens with the antiestrogen nafoxidine blocked the subsequent increase in adherence. These data suggest that estrogen receptors are present in HeLa cells and that hormonally-induced alterations in the synthesis of bacterial receptor sites may modify the capacity of certain cells to bind bacteria.
机译:用增加浓度的雌激素孵育融合细胞培养的HeLa细胞18小时,逐渐增强了各种放射性标记细菌对HeLa细胞的附着。此作用不是由其他激素引起的,也不是通过将HeLa细胞或细菌与激素孵育1小时而产生的。雌激素没有类似地影响所研究的另外两个受体细胞系。添加代谢抑制剂表明,雌激素对HeLa细胞的这种作用是能量依赖性的,并涉及蛋白质的合成。 HeLa细胞和雌激素与抗雌激素萘那汀同时孵育可阻止随后的依从性增加。这些数据表明,雌激素受体存在于HeLa细胞中,激素诱导的细菌受体位点合成中的改变可能会改变某些细胞与细菌结合的能力。

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