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首页> 外文期刊>Infection and immunity >Identification of an antigen-specific immunoglobulin M antibody associated with acute Toxoplasma infection.
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Identification of an antigen-specific immunoglobulin M antibody associated with acute Toxoplasma infection.

机译：鉴定与急性弓形虫感染有关的抗原特异性免疫球蛋白M抗体。

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We used the protein gel transfer technique (Western blotting) to analyze the immune response to individual electrophoretically resolved Toxoplasma antigens. Toxoplasma antigens were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The array of resolved proteins was electrophoretically transferred to CNBr-activated filter paper and incubated with serum samples from patients with either acute or chronic Toxoplasma infections or with serum samples from seronegative controls. Serum immunoglobulin G and immunoglobulin M bound to individual Toxoplasma antigens were detected by radioiodinated staphylococcal protein A and a goat anti-human immunoglobulin M antibody, respectively. A complex array of Toxoplasma antigens was revealed by this procedure. Immunoglobulin M directed against a low-molecular-weight antigen was detected in sera from all acutely infected adults (19 sera tested) and was absent from all sera from individuals with chronic infections (9 sera tested) and from sera from seronegative controls (9 sera tested).
机译：我们使用蛋白质凝胶转移技术（蛋白质印迹）来分析对电泳分离的弓形虫抗原的免疫应答。弓形虫抗原通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离。分离的蛋白质阵列通过电泳转移到CNBr激活的滤纸上，并与急性或慢性弓形虫感染患者的血清样品或血清阴性对照的血清样品一起孵育。分别通过放射性碘化的葡萄球菌蛋白A和山羊抗人免疫球蛋白M抗体检测结合到弓形虫抗原的血清免疫球蛋白G和免疫球蛋白M。该程序揭示了复杂的弓形虫抗原阵列。在所有急性感染的成年人的血清中检测到了针对低分子量抗原的免疫球蛋白M（已测试19份血清），而在慢性感染患者的所有血清中均未检测到该血清（已测试9份血清），而血清阴性对照的血清中未检测到（9份血清）经过测试）。

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