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首页> 外文期刊>Infection and immunity >Effective immunity to dental caries: gastric intubation of Streptococcus mutans whole cells or cell walls induces protective immunity in gnotobiotic rats.
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Effective immunity to dental caries: gastric intubation of Streptococcus mutans whole cells or cell walls induces protective immunity in gnotobiotic rats.

机译：对龋齿的有效免疫力：变形链球菌全细胞或细胞壁的胃插管可在致生性大鼠中诱导保护性免疫力。

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Gnotobiotic rats were given Streptococcus mutans 6715 whole cells (WC), purified cell walls (CW), or cell wall lysate by gastric intubation (GI), and assessments were made of humoral immune responses in serum and saliva and of caries protection. Levels of secretory immunoglobulin A (IgA) and IgG antibodies to S. mutans WC in saliva samples from experimental rats were determined by an enzyme-linked immunosorbent assay. Serum antibody levels of the IgM, IgG, and IgA isotypes were also determined. Similar levels of salivary antibodies were induced in rats given S. mutans WC or CW by GI, whereas lower salivary antibody titers were observed in rats given cell wall lysate by the oral route. The level of serum antibodies in the various groups of rats also reflected the oral antigen used. The specificity of salivary IgA and serum IgG antibodies in the various groups of rats was determined by enzyme-linked immunosorbent assay with lipoteichoic acid, serotype g carbohydrate, dextran, CW, and WC as coating antigens. Salivary IgA and serum IgG antibodies in rats given S. mutans WC or CW by GI were primarily directed to lipoteichoic acid and serotype g carbohydrate. The presence of salivary IgA antibodies to S. mutans in rats given either S. mutans WC or CW by GI correlated with a significant reduction in the levels of plaque, numbers of viable S. mutans in plaque, and caries scores when compared with the control animals (infected only). These results demonstrate that particulate antigens of S. mutans induce salivary immune responses when given by GI to gnotobiotic rats and that the presence of these antibodies correlates with caries protection.
机译：灵长类动物大鼠通过胃插管（GI）获得了变形链球菌6715全细胞（WC），纯化的细胞壁（CW）或细胞壁裂解物，并评估了血清和唾液中的体液免疫反应以及龋齿保护。通过酶联免疫吸附测定法测定实验大鼠唾液样品中分泌的免疫球蛋白A（IgA）和抗变形链球菌WC的IgG抗体水平。还确定了IgM，IgG和IgA同种型的血清抗体水平。在通过胃肠道给予变形链球菌WC或CW的大鼠中，唾液抗体水平相似，而在口服细胞壁裂解液的大鼠中，唾液抗体滴度较低。各组大鼠中血清抗体的水平也反映了所用的口服抗原。唾液IgA和血清IgG抗体在各组大鼠中的特异性是通过脂蛋白酸，血清型g碳水化合物，右旋糖酐，CW和WC作为包被抗原的酶联免疫吸附试验确定的。胃肠道给予变形链球菌WC或CW的大鼠的唾液IgA和血清IgG抗体主要针对脂磷壁酸和血清型g碳水化合物。与对照组相比，通过胃肠道给予变形链球菌WC或CW的大鼠唾液中存在针对变形链球菌的IgA抗体，与噬菌斑水平，噬菌斑中存活的变形链球菌数量和龋齿评分的显着降低有关。动物（仅受感染）。这些结果表明，变形链球菌的颗粒抗原当由胃肠道给予致生素大鼠时诱导唾液免疫反应，并且这些抗体的存在与龋齿保护相关。

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