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首页> 外文期刊>Infection and immunity >Ribosomal protein phosphorylation induced during Q fever or by lipopolysaccharide: in vitro translation is stimulated by infected liver ribosomes.
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Ribosomal protein phosphorylation induced during Q fever or by lipopolysaccharide: in vitro translation is stimulated by infected liver ribosomes.

机译:Q发热或脂多糖诱导的核糖体蛋白磷酸化:受感染的肝核糖体刺激体外翻译。

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Q fever, as well as the lipopolysaccharide prepared from the rickettsial agent Coxiella burnetii, stimulates the phosphorylation of guinea pig liver ribosomal protein S6. In vitro mRNA and ribosome-dependent rabbit reticulocyte lysate translation systems reconstituted with ribosomes and mRNAs from infected animal livers were more active than those with mRNAs and ribosomes from uninfected animals. Treatment of ribosomes with a ribosomal supernatant phosphatase reduced the in vitro translation activities; the largest decreases occurred in systems with ribosomes and mRNAs from infected liver. These experiments provide a basis for explaining the increased hepatic protein synthesis during Q fever and demonstrate, perhaps for the first time, the phosphorylation of ribosomal protein in response to lipopolysaccharide. The implications of these observations are discussed in the context of previous studies on stimulated transcription and translation during Q fever.
机译:Q热以及由立克次氏体枯草杆菌制备的脂多糖刺激豚鼠肝核糖体蛋白S6的磷酸化。用核糖体重建的体外mRNA和依赖核糖体的兔网状细胞裂解物翻译系统,感染动物肝脏的mRNA比未感染动物的mRNA和核糖体的活性更高。用核糖体上清磷酸酶处理核糖体会降低体外翻译活性。最大的下降发生在带有被感染肝脏的核糖体和mRNA的系统中。这些实验为解释Q发烧期间肝蛋白合成增加提供了基础,并可能首次证明了核糖体蛋白对脂多糖的磷酸化。这些观察结果的含义在先前关于Q发烧期间受激转录和翻译的研究中进行了讨论。

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