...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting.
【24h】

Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting.

机译：poly虫立克次体的多肽组成和抗原成分的聚丙烯酰胺凝胶电泳和免疫印迹分析。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Polyacrylamide gel electrophoresis of lysates of purified Rickettsia tsutsugamushi revealed as many as 30 polypeptide bands, including major bands corresponding to molecular sizes of 70, 60, 54 to 56, and 46 to 47 kilodaltons. Compared with the polypeptide composition of the rickettsiae of Gilliam, Karp, and Kato strains and a newly isolated Shimokoshi strain, the major polypeptide in the Kato strain (54-56K) and in the Karp strain (46-47K) migrated a little faster and slower, respectively, than the corresponding polypeptides in the other strains. The largest major polypeptide (54-56K) was digestible by the treatment of intact rickettsiae with trypsin and variable in content in separate preparations, suggesting that the polypeptide exists on the rickettsial surface and is easily degraded during the handling of these microorganisms. Several surface polypeptides of rickettsiae, including the 54-56K and 46-47K polypeptides, were detected by radioiodination of intact rickettsiae followed by polyacrylamide gel electrophoresis of the lysate; however, the 70K and 60K polypeptides were not labeled. Immunoblotting experiments with hyperimmune sera prepared in guinea pigs against each strain demonstrated that the 70K, 54-56K, and 46-47K polypeptides showed antigenic activities. The 54-56K polypeptide appeared to be strain specific, whereas the 70K and 46-47K polypeptides cross-reacted with the heterologous antisera.
机译：纯化的tsu虫立克次体的裂解物的聚丙烯酰胺凝胶电泳显示多达30条多肽条带，包括对应于70、60、54至56和46至47千道尔顿分子大小的主要条带。与Gilliam，Karp和Kato菌株的立克次体和新分离的Shimokoshi菌株的多肽组成相比，Kato菌株（54-56K）和Karp菌株（46-47K）中的主要多肽迁移更快，并且分别比其他菌株中的相应多肽慢。最大的主要多肽（54-56K）可通过用胰蛋白酶处理完整的立克次体来消化，并且其含量在不同的制剂中存在差异，这表明该多肽存在于立克次体表面，在处理这些微生物时很容易降解。通过对完整的立克次体进行放射性碘化，然后对裂解物进行聚丙烯酰胺凝胶电泳，检测到了立克次体的几种表面多肽，包括54-56K和46-47K多肽。然而，70K和60K多肽未标记。用豚鼠针对每种病毒株制备的超免疫血清进行的免疫印迹实验表明，70K，54-56K和46-47K多肽具有抗原活性。 54-56K多肽似乎是菌株特异性的，而70K和46-47K多肽则与异源抗血清发生交叉反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号