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首页> 外文期刊>Infection and immunity >Effect of crevicular fluid and lysosomal enzymes on the adherence of streptococci and bacteroides to hydroxyapatite.
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Effect of crevicular fluid and lysosomal enzymes on the adherence of streptococci and bacteroides to hydroxyapatite.

机译：宫颈液和溶酶体酶对链球菌和类细菌对羟磷灰石粘附的影响。

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Samples of hydroxyapatite (HA) beads smaller than 1 mg were coated with 10 microliter of either saliva, serum, or human crevicular fluid before being added to a suspension of Streptococcus sanguis or Bacteroides gingivalis. In some assays, preparations of a granular fraction, elastase, or cathepsin G from human leukocytes were used to coat HA or to treat saliva-coated HA (SHA) before mixing with bacteria. The number of cells adhering to the beads was then counted under a scanning electron microscope by a standardized procedure. More cells were found to adhere to SHA in this assay than in the conventional large-scale assay. Human crevicular fluid, even when diluted up to three times, completely inhibited the adherence of S. sanguis to HA. A 100% inhibition of S. sanguis adherence was also observed when HA was coated with the granular fraction of leukocytes, and a 65% inhibition observed when SHA was treated with the enzyme preparation. When used to coat HA, elastase and cathepsin G reduced the adherence of S. sanguis by 30 and 50%, respectively. The binding of S. sanguis to elastase- or cathepsin G-treated SHA was also reduced. B. gingivalis 33277 was found to adhere in high numbers to SHA. Coating HA with crevicular fluid or with the lysosomal enzyme preparation had a limited negative effect. We postulate that crevicular fluid prevents the adherence of S. sanguis by virtue of either its enzyme content or its albumin content or both.
机译：小于1毫克的羟磷灰石（HA）珠​​样品在被添加到血链球菌或牙龈细菌之前，要用10微升的唾液，血清或人的卵泡液包被。在某些测定中，在与细菌混合之前，使用人白细胞中的颗粒级分，弹性蛋白酶或组织蛋白酶G的制剂包被HA或治疗唾液包被的HA（SHA）。然后，通过标准化程序在扫描电子显微镜下计数粘附到珠子上的细胞数。与常规大规模测定法相比，在该测定法中发现更多的细胞粘附于SHA。即使稀释至三倍，人的宫颈液也完全抑制了血gui对HA的粘附。当HA用白细胞的颗粒部分包被时，还观察到血红葡萄球菌粘附的100％抑制，而当用酶制剂处理SHA时，观察到65％的抑制。当用于包被HA时，弹性蛋白酶和组织蛋白酶G分别使血红链球菌的附着力降低30％和50％。血链球菌与弹性蛋白酶或组织蛋白酶G处理的SHA的结合也降低了。发现牙龈芽孢杆菌33277大量粘附于SHA。用宫颈液或溶酶体酶制剂包被HA的负面影响有限。我们推测，由于其酶含量或白蛋白含量或两者兼而有之，因此，子宫颈液阻止了桑氏葡萄球菌的粘附。

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