...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Enzymatic and antigenic characterization of immunoglobulin A1 proteases from Bacteroides and Capnocytophaga spp.
【24h】

Enzymatic and antigenic characterization of immunoglobulin A1 proteases from Bacteroides and Capnocytophaga spp.

机译：拟杆菌和Capnocytophaga spp的免疫球蛋白A1蛋白酶的酶和抗原表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacteroides and Capnocytophaga species have been implicated as periodontal pathogens. Some of these species possess immunoglobulin A1 (IgA1) proteases that are capable of cleaving the human IgA1 molecule in the hinge region, leaving intact Fc alpha and Fab alpha fragments. The purpose of this study was to characterize this activity. In addition to IgA1 protease activity in already known species, IgA1 protease activity was a feature of Bacteroides buccalis, Bacteroides oralis, Bacteroides veroralis, Bacteroides capillus, and Bacteroides pentosaceus. Results of immunoelectrophoretic and sodium dodecyl sulfate-polyacrylamide gel electrophoretic analyses suggested that all species cleave the alpha-chain at the same peptide bond, i.e., the prolyl-seryl bond between residues 223 and 224 in the hinge region. The Bacteroides proteases could be classified as thiol proteases, which were at the same time dependent on metal ions, while the Capnocytophaga proteases were metallo enzymes. None of the proteases were inhibited by the physiologic proteases inhibitors alpha 2-macroglobulin and alpha 1-proteinase inhibitor. Investigations with enzyme-neutralizing antibodies raised in rabbits against protease preparations from the respective type strains revealed that, despite otherwise identical characteristics, the IgA1 protease of each Bacteroides species was antigenically distinct. Bacteroides buccae and the two later synonymous species B. capillus and B. pentosaceus produced identical proteases. In contrast, IgA1 proteases from Capnocytophaga ochracea and Capnocytophaga sputigena strains were apparently identical, while Capnocytophaga gingivalis had a protease that differed from those of the other Capnocytophaga species.
机译：拟杆菌属和Capnocytophaga物种已被认为是牙周病原体。这些物种中的某些具有免疫球蛋白A1（IgA1）蛋白酶，能够在铰链区切割人IgA1分子，而保留完整的Fcα和Fabα片段。这项研究的目的是表征这种活动。除了已知物种中的IgA1蛋白酶活性外，IgA1蛋白酶活性也是颊细菌杆菌，口腔拟杆菌，口生细菌，角膜拟杆菌和戊齿拟杆菌的特征。免疫电泳和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳的结果表明，所有物种均在相同的肽键（即铰链区中第223和224位残基之间的脯氨酰-丝氨酰键）处裂解α链。拟杆菌属蛋白酶可分类为硫醇蛋白酶，其同时依赖于金属离子，而Capnocytophaga蛋白酶则为金属酶。生理蛋白酶抑制剂α2-巨球蛋白和α1蛋白酶抑制剂均不抑制蛋白酶。用家兔针对来自各类型毒株的蛋白酶制剂产生的中和酶的抗体进行的研究表明，尽管具有其他相同的特征，但每个拟杆菌属的IgA1蛋白酶在抗原上是不同的。 bucceroides buccae和后来的两个同义物种B. capillus和B. pentosaceus产生相同的蛋白酶。相反，来自Capnocytophaga ochracea和Sputigena sputigena菌株的IgA1蛋白酶显然是相同的，而齿龈Capnocytophaga gingivalis的蛋白酶不同于其他Capnocytophaga物种。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号