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首页> 外文期刊>Infection and immunity >Antibody responses to Toxoplasma gondii in sera, intestinal secretions, and milk from orally infected mice and characterization of target antigens.
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Antibody responses to Toxoplasma gondii in sera, intestinal secretions, and milk from orally infected mice and characterization of target antigens.

机译:口腔感染小鼠对弓形虫的血清,肠道分泌物和牛奶的抗体反应以及靶抗原的鉴定。

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Toxoplasma gondii-specific antibody responses in serum, intestinal secretions, and milk were identified with an enzyme-linked immunosorbent assay following a single oral infection of mice with strain 76K cysts of T. gondii. Immunoglobulin A (IgA) production began during week 2 of infection in serum and milk and during week 3 of infection in intestinal secretions and persisted in all three throughout the experiment (17 weeks). IgG but not IgM antibodies were detected in intestinal secretions later in the infection. Serum and milk IgG and IgM production began at the same time after infection as did the IgA response. In Western blotting (immunoblotting), intestinal IgA antibodies were shown to react with antigens comigrating with the T. gondii proteins p22, p23, p30, and p43, the 28-kilodalton antigen, and the 55- and 60-kilodalton rhoptry proteins, as recognized by specific monoclonal antibodies. Milk IgA antibodies reacted with antigens comigrating with p30 and p43. Most of the antigens recognized by IgA antibodies were also detected by IgG antibodies. IgA antibodies from all three biological samples detected the same major T. gondii antigens; thus, there was apparently no specific antibody production unique to one locality.
机译:在刚感染刚地弓形虫菌株76K的小鼠单次口服感染后,通过酶联免疫吸附法鉴定了血清,肠道分泌物和牛奶中的弓形虫特异性抗体反应。免疫球蛋白A(IgA)的产生在血清和牛奶感染的第2周开始,肠道分泌物的感染在第3周开始,并在整个实验(17周)中持续存在。感染后肠道分泌物中检测到IgG,但未检测到IgM抗体。感染后血清和牛奶中的IgG和IgM的产生与IgA反应一样开始。在Western印迹法(免疫印迹)中,肠IgA抗体显示出与刚地弓形虫蛋白p22,p23,p30和p43、28千道尔顿抗原以及55以及60千道尔顿rhoptry蛋白相关的抗原反应。被特异性单克隆抗体识别。牛奶IgA抗体与p30和p43的抗原反应。 IgA抗体识别的大多数抗原也被IgG抗体检测到。来自所有三个生物学样品的IgA抗体检测到相同的主要弓形虫抗原;因此,显然没有一个地方特有的特异性抗体产生。

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