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Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates.

机译:选择性识别溶组织变形杆菌的子集的单克隆抗体的表征。

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Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.
机译:单克隆抗体2D7.10识别一种抗原,该抗原存在于九种无菌培养的解组织变形虫中,其中七种存在,而在所有其他研究的变形虫中均不存在。该抗原在两种分离株中不存在:200:NIH和Rahman。所有九种分离物均属于致病性酶Ⅱ。蛋白质印迹(免疫印迹)分析和高碘酸盐和蛋白水解酶胰蛋白酶的处理表明2D7.10识别的抗原是碳水化合物部分。

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