• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Oral immunization of mice with a live recombinant Yersinia enterocolitica O:9 strain that produces the cholera toxin B subunit.
【24h】

Oral immunization of mice with a live recombinant Yersinia enterocolitica O:9 strain that produces the cholera toxin B subunit.

机译：用产生霍乱毒素B亚基的活重组小肠结肠炎耶尔森菌O：9菌株对小鼠进行口服免疫。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The 70-kilobase pYV plasmid of Yersinia enterocolitica encodes a set of proteins called Yops that are produced during infection. To use Y. enterocolitica as a live carrier to present the cholera toxin B (CT-B) subunit to the immune system, we constructed an operon fusion between ctxB and the yop51 gene. This operon fusion was either cloned on an RSF1010-derived plasmid or integrated into the pYV plasmid itself. In Y. enterocolitica, both constructions directed the synthesis of free CT-B only under conditions of Yops production, i.e., at 37 degrees C in a medium deprived of Ca2+. Bacteria containing both types of recombinant plasmids were given orally to mice. A serum antibody response against CT-B was detected in both cases. A secretory immunoglobulin A activity specific to CT-B was also observed in the intestinal secretions. According to immunoblot analysis, the serum antibody response was only directed against the polymeric form of the B subunit. The ctxB gene was also inserted in frame within yop51, giving a chimeric Yop51-CT-B protein that was secreted into the surrounding medium. In this case, however, no antibody response was observed after oral inoculation of mice. This lack of response probably results from the inability of the hybrid protein to assemble into the polymeric form of the B subunit.
机译：小肠结肠炎耶尔森氏菌的70碱基对pYV质粒编码一组在感染过程中产生的称为Yops的蛋白质。为了使用小肠结肠炎耶尔森菌作为活载体将霍乱毒素B（CT-B）亚基呈递给免疫系统，我们构建了ctxB和yop51基因之间的操纵子融合体。该操纵子融合体可克隆到RSF1010衍生的质粒上或整合到pYV质粒本身中。在小肠结肠炎耶尔森氏菌中，两种构造仅在Yops产生的条件下，即在37℃下在缺乏Ca 2+的培养基中指导游离CT-B的合成。将包含两种类型的重组质粒的细菌口服给予小鼠。在两种情况下均检测到针对CT-B的血清抗体反应。在肠分泌物中也观察到了对CT-B有特异性的分泌性免疫球蛋白A活性。根据免疫印迹分析，血清抗体应答仅针对B亚基的聚合形式。 ctxB基因也被插入yop51的框架中，产生了嵌合的Yop51-CT-B蛋白，该蛋白被分泌到周围的培养基中。然而，在这种情况下，小鼠口服接种后未观察到抗体反应。这种缺乏应答的原因可能是由于杂合蛋白无法组装成B亚基的聚合形式。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号