...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Investigation of the Role of Type IVAeromonas Pilus (Tap) in the Pathogenesis ofAeromonas Gastrointestinal Infection
【24h】

Investigation of the Role of Type IVAeromonas Pilus (Tap) in the Pathogenesis ofAeromonas Gastrointestinal Infection

机译：IVA型肺炎小球菌（Tap）在气单胞菌胃肠道感染的发病机制中的作用的调查。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although there is substantial evidence that type IV pili purified from diarrhea-associated Aeromonas species (designated Bfp for bundle-forming pilus) are intestinal colonization factors (S. M. Kirov, L. A. O'Donovan, and K. Sanderson, Infect. Immun. 67:5447–5454, 1999), nothing is known regarding the function of a second family of Aeromonas type IV pili (designated Tap for type IV Aeromonas pilus), identified following the cloning of a pilus biogenesis gene cluster tapABCD. Related pilus gene clusters are widely conserved among gram-negative bacteria, but their significance for virulence has been controversial. To investigate the role of Tap pili in Aeromonas pathogenesis, mutants ofAeromonas strains (a fish isolate of A. hydrophila and a human dysenteric isolate of A. veronii bv. sobria) were prepared by insertional inactivation of the tapA gene which encodes the type IV pilus subunit protein, TapA. Exotoxic activities were unaffected by the mutation intapA. Inactivation of tapA had no effect on the bacterial adherence of these two isolates to HEp-2 cells. For theA. veronii bv. sobria isolate, adhesion to Henle 407 intestinal cells and to human intestinal tissue was also unaffected. There was no significant effect on the duration of colonization or incidence of diarrhea when the A. veronii bv. sobria strain was tested in the removable intestinal tie adult rabbit diarrhea model or on its ability to colonize infant mice. Evidence was obtained that demonstrated that TapA was expressed by both Aeromonasspecies and was present on the cell surface, although if assembled into pili this pilus type appears to be an uncommon one under standard bacterial growth conditions. Further studies into factors which may influence Tap expression are required, but the present study suggests that Tap pili may not be as significant as Bfp pili forAeromonas intestinal colonization.
机译：尽管有充分的证据表明从腹泻相关的气单胞菌物种（命名为Bfp的成束菌毛）纯化的IV型菌毛是肠道定居因子（SM Kirov，LA O'Donovan和K.Sanderson，Infect。Immun。67：5447）。 ––5454，1999），关于克隆第四代菌毛生物发生基因簇tapABCD后鉴定的第二型第四代气单胞菌菌毛（命名为第四型气单胞菌菌丝）的功能尚不清楚。相关菌毛基因簇在革兰氏阴性细菌中被广泛保存，但是它们对毒力的重要性一直存在争议。为了研究Tap菌毛在气单胞菌发病机理中的作用，通过插入失活编码IV型菌毛的tapA基因，制备了气单胞菌菌株的突变体（嗜水气单胞菌的鱼分离物和维罗尼氏菌的人痢疾分离物）。亚基蛋白TapA。外毒素活性不受突变intapA的影响。 tapA的失活对这两个分离株对HEp-2细胞的细菌粘附没有影响。对于A。维罗尼公司分离出的霍乱弧菌，对Henle 407肠细胞和对人肠组织的粘附也未受影响。 ver.ii.veronii bv。的定植时间或腹泻发生率没有明显影响。在可移动肠成年兔腹泻模型中或对它定殖在婴儿小鼠身上的能力中测试了sobria菌株。获得的证据表明，TapA由两种气单胞菌物种表达并且存在于细胞表面，尽管如果组装成菌毛，则这种菌毛类型在标准细菌生长条件下似乎是不常见的。需要进一步研究可能影响Tap表达的因素，但是本研究表明Tap pili可能不如Bfp pili在气单胞菌肠道定殖中具有重要意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号