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首页> 外文期刊>Infection and immunity >Expression of Surfactant Protein D in the Human Gastric Mucosa and during Helicobacter pylori Infection
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Expression of Surfactant Protein D in the Human Gastric Mucosa and during Helicobacter pylori Infection

机译：表面活性蛋白D在人胃黏膜和幽门螺杆菌感染过程中的表达

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摘要

Helicobacter pylori establishes persistent infection of gastric mucosa with diverse clinical outcomes. The innate immune molecule surfactant protein D (SP-D) binds selectively to microorganisms, inducing aggregation and phagocytosis. In this study, we demonstrated the expression of SP-D in gastric mucosa by reverse transcription-PCR and immuohistochemical analysis. SP-D is present at the luminal surface and within the gastric pits, with maximal expression at the surface. Levels of expression are significantly increased in H. pylori-associated gastritis compared to those in the normal mucosa. Immunofluorescence microscopy was used to demonstrate binding and agglutination of H. pylori by SP-D in a lectin-specific manner. These activities resulted in a 50% reduction in the motility of H. pylori, as judged on the basis of curvilinear velocity measured by using a Hobson BacTracker. Lipopolysaccharides extracted from three H. pylori strains were shown to bind SP-D in a concentration-dependent manner, and there was marked variation in the avidity of binding among the strains. SP-D may therefore play a significant role in the innate immune response to H. pylori infection.
机译： Helicobacter pylori 建立了胃黏膜的持续感染，临床结果多种多样。先天性免疫分子表面活性剂蛋白D（SP-D）选择性结合微生物，诱导聚集和吞噬作用。在这项研究中，我们通过逆转录PCR和免疫组化分析证明了SP-D在胃粘膜中的表达。 SP-D存在于腔表面和胃腔内，在表面具有最大表达。在 H中表达水平显着增加。与正常黏膜中的幽门螺杆菌相关的胃炎。免疫荧光显微镜用于证明 H的结合和凝集。 SP-D产生的幽门螺杆菌特异性凝集素。这些活动导致 H的活力降低了50％。幽门螺旋杆菌，根据使用Hobson BacTracker测量的曲线速度进行判断。从三个 H中提取的脂多糖。显示幽门螺杆菌菌株以浓度依赖性方式结合SP-D，并且菌株之间的结合亲和力存在显着差异。因此，SP-D可能在对H的先天免疫应答中起重要作用。幽门螺杆菌感染。

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