Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens.

M R Halter; L A Joens

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Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens.

机译：来自猪痢疾密螺旋体和无毒螺旋体的脂寡糖。

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Lipooligosaccharides from Treponema hyodysenteriae serotypes 1 through 7, attenuated T. hyodysenteriae serotypes 1 and 2, and five strains of T. innocens were extracted with hot phenol water. The extracts were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation and analyzed by lipopolysaccharide selective silver staining and Western blot (immunoblot) immunodetection. Silver staining revealed the presence of two bands that ranged between 18,000 and 24,000 daltons and that were serotype specific for T. hyodysenteriae. Attenuation of pathogenic strains resulted in the loss of the higher-molecular-weight band. Four of five T. innocens strains also lacked this particular band. T. innocens 421 had six bands between 17,000 and 26,900 daltons. Western blots with hyperimmune rabbit sera and convalescent-phase swine sera revealed antigenic variation among serotypes of T. hyodysenteriae and attenuated serotypes of T. hyodysenteriae. Convalescent-phase swine sera failed to recognize lipopolysaccharides from T. innocens. Differences in results obtained by lipopolysaccharide selective silver staining versus immunoblotting of the lipopolysaccharide preparations probably indicate that these two methods identify separate characteristics of the same molecule.

机译：用热酚水提取猪痢疾密螺旋体血清型1至7，减毒猪痢疾螺旋体血清型1和2中的脂寡糖和五株无性疟原虫。对提取物进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离，并通过脂多糖选择性银染和Western blot（免疫印迹）免疫检测进行分析。银染显示存在两个条带，分别在18,000和24,000道尔顿之间，并且分别是猪痢疾短螺旋体的血清型。致病菌株的衰减导致高分子量谱带的丧失。五种天竺葵（T.nonocens）菌株中有四株也缺乏该特定谱带。 T. innocens 421有6个波段，介于17,000和26,900道尔顿之间。用超免疫兔血清和恢复期猪血清进行的蛋白质印迹显示猪痢疾短螺旋体血清型和猪痢疾短螺旋体血清型之间的抗原变异。恢复期的猪血清无法识别来自无病菌的脂多糖。通过脂多糖选择性银染色与脂多糖制备物的免疫印迹获得的结果差异可能表明这两种方法可鉴定同一分子的不同特征。

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《Infection and immunity》 |1988年第12期|共5页
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M R Halter; L A Joens;
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1. Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens. [J] . M R Halter, L A Joens Infection and immunity . 1988,第12期

机译：来自猪痢疾密螺旋体和无毒螺旋体的脂寡糖。
2. Comparison of the biological responses induced by lipopolysaccharide and endotoxin of Treponema hyodysenteriae and Treponema innocens. [J] . J M Greer, M J Wannemuehler Infection and immunity . 1989,第3期

机译：猪痢疾密螺旋体和无毒螺旋体脂多糖和内毒素诱导的生物反应的比较。
3. Identification of the major antigens of Treponema hyodysenteriae and comparison with those of Treponema innocens. [J] . S N Chatfield, D S Fernie, C Penn, Infection and immunity . 1988,第5期

机译：鉴定猪痢疾密螺旋体的主要抗原，并与无毒螺旋体进行比较。
4. Evaluation of a Ciprofloxacin and Metronidazole Encapsulated Biomimetic Nanomatrix Gel on Enterococcus faecalis and Treponema denticola [C] . Sagar N. Kaushik, Taneidra Walker, Jae-Hong Park, Society for Biomaterials annual meeting and exposition . 2015

机译：环丙沙星和甲硝唑封装的仿生纳米基质凝胶对粪肠球菌和密螺旋体的评价
5. EPIDEMIOLOGY OF SWINE DYSENTERY: DEVELOPMENT AND EVALUATION OF CULTURAL AND SEROLOGICAL METHODS FOR THE DETECTION OF TREPONEMA HYODYSENTERIAE INFECTION. [D] . SONGER, JOSEPH GLENN. 1976

机译：猪痢疾的流行病学：用于检测丙型肝炎性小肠痢疾感染的文化和血清学方法的发展与评估。
6. Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens. [O] . M R Halter, L A Joens 1988

机译：猪痢疾密螺旋体和无毒螺旋体的脂寡糖。
7. Reclassification of Treponema hyodysenteriae and Treponema innocens in a New Genus, Serpula gen. nov., as Serpula hyodysenteriae comb. nov. and Serpula innocens comb. nov. [O] . T. B. STANTON, N. S. JENSEN, T. A. CASEY, 1991

机译：在新属，裂纹血清术中的粒细胞静脉内塞内特芹素和Treponema无辜的重新分类。 11月。，作为Serpula Hyodysenteriae梳子。 11月。和Serpula无辜的梳子。 11月。

Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens.

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