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首页> 外文期刊>Infection and immunity >Antigenic analysis of Campylobacter species and an intracellular Campylobacter-like organism associated with porcine proliferative enteropathies.
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Antigenic analysis of Campylobacter species and an intracellular Campylobacter-like organism associated with porcine proliferative enteropathies.

机译：弯曲杆菌种和与猪增生性肠病相关的细胞内弯曲杆菌样生物的抗原分析。

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摘要

Whole-cell and outer membrane preparations of Campylobacter mucosalis, C. hyointestinalis, C. jejuni, and C. coli isolated from porcine intestines were compared with preparations of intracellular Campylobacter-like organisms extracted directly from the lesions of pigs with proliferative enteropathy. By gradient polyacrylamide gel electrophoresis, outer membrane and total protein profiles of C. mucosalis, C. hyointestinalis, C. jejuni, and C. coli were significantly different from each other and from those of the Campylobacter-like organisms. Immunoblotting of these preparations with rabbit antisera or monoclonal antibodies prepared against the intracellular Campylobacter-like organisms showed strong reactions only with a 25,000- to 27,000-molecular-weight component of preparations of the intracellular organisms. Antisera to cultivable Campylobacter species isolates did not react with preparations of intracellular organisms. Isoelectric focusing of sonicated preparations showed protein profile differences and an immune-reactive component in the intracellular organisms with a pI of 4.5. This study suggests that the intracellular Campylobacter-like organism associated with proliferative enteropathy may be a novel bacterium with significant antigenic differences from the Campylobacter species previously associated with the disease.
机译：比较了从猪肠分离的粘膜弯曲杆菌，豚肠弯曲杆菌，空肠弯曲杆菌和大肠杆菌的全细胞和外膜制剂，以及直接从增生性肠病猪病变中提取的细胞内弯曲杆菌样生物制剂。通过梯度聚丙烯酰胺凝胶电泳，粘膜梭状芽胞杆菌，豚肠弯曲杆菌，空肠弯曲杆菌和大肠杆菌的外膜和总蛋白谱彼此之间以及与弯曲杆菌样生物之间存在显着差异。用兔抗血清或针对细胞内弯曲杆菌样生物制备的单克隆抗体对这些制剂进行免疫印迹显示，仅与细胞内生物制剂的25,000至27,000分子量的组分发生强烈反应。可培养弯曲杆菌属菌株的抗血清不与细胞内生物制剂反应。超声处理过的制剂的等电聚焦显示细胞内生物中的蛋白质谱差异和免疫反应成分，pI为4.5。这项研究表明，与增生性肠病有关的细胞内弯曲杆菌样生物可能是一种新型细菌，与以前与该疾病有关的弯曲杆菌具有明显的抗原差异。

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