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首页> 外文期刊>Infection and immunity >Identification of Pneumocystis carinii chromosomes and mapping of five genes.
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Identification of Pneumocystis carinii chromosomes and mapping of five genes.

机译：卡氏肺孢子虫染色体的鉴定和五个基因的作图。

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Pulsed field gel electrophoresis was used to identify the chromosome-size DNA of Pneumocystis carinii, a major pathogen of immunocompromised patients. Thirteen chromosomes of rodent Pneumocystis carinii, ranging in size from 300 to 700 kilobases (kb), were identified. The minimum genome size for P. carinii, estimated on the basis of the sizes of chromosomes, is 7,000 kb. Genetic heterogeneity among different P. carinii isolates was documented by demonstration of chromosomal size variability. By hybridization studies, the genes for topoisomerase I, dihydrofolate reductase, rRNA, actin, and thymidylate synthase were mapped to single chromosomes of approximately 650, 590, 550, 460, and 350 kb, respectively. Hybridization studies further confirmed the genetic heterogeneity of P. carinii.
机译：脉冲场凝胶电泳用于鉴定卡氏肺孢子虫（Pneumocystis carinii）的染色体大小的DNA，卡氏肺孢子虫是免疫功能低下患者的主要病原体。确定了啮齿动物肺孢子虫的13条染色体，大小从300到700千碱基（kb）不等。根据染色体大小估算的卡氏疟原虫最小基因组大小为7,000 kb。通过证明染色体大小可变性，证明了不同卡氏毕赤酵母分离株之间的遗传异质性。通过杂交研究，拓扑异构酶I，二氢叶酸还原酶，rRNA，肌动蛋白和胸苷酸合酶的基因分别定位在大约650、590、550、460和350 kb的单条染色体上。杂交研究进一步证实了卡氏假单胞菌的遗传异质性。

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