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首页> 外文期刊>Infection and immunity >Genetic and immunological analysis of Mycobacterium tuberculosis fibronectin-binding proteins.
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Genetic and immunological analysis of Mycobacterium tuberculosis fibronectin-binding proteins.

机译：结核分枝杆菌纤连蛋白结合蛋白的遗传和免疫学分析。

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Recombinant phage clones, TB1 and TB2, were selected from a Mycobacterium tuberculosis lambda gt11 DNA expression library by screening with a polyclonal antiserum raised against the antigen 85 complex of Mycobacterium bovis BCG. Analysis of recombinant DNA inserts and expressed fusion proteins showed that two new genes had been isolated. The product of clone TB2 was identified as a member of the 30/31-kDa antigen 85 complex. Restriction enzyme analysis showed that this gene differs from previously cloned members of this antigen complex, with detailed serological analysis indicating that it may encode the 85C component. Antisera raised against the expressed product of clone TB1 recognized a 55-kDa protein in M. tuberculosis extracts. The 55-kDa protein also has fibronectin-binding activity and, like the 30/31-kDa family, is a prominent target of the antibody response in patients with mycobacterial disease. Although the clones were selected by using the same antiserum, detailed analysis by serology and by DNA hybridization showed that they represent two quite distinct types of fibronectin-binding activities expressed by M. tuberculosis. Further analysis of the fibronectin-binding antigens of M. tuberculosis may provide important insights into their role in mediating the interaction with the host immune system.
机译：通过用针对牛分枝杆菌BCG抗原85复合物的多克隆抗血清筛选，从结核分枝杆菌λgt11DNA表达文库中选择重组噬菌体克隆TB1和TB2。重组DNA插入片段和表达的融合蛋白的分析表明已分离出两个新基因。克隆TB2的产物被鉴定为30 / 31-kDa抗原85复合物的成员。限制性内切酶分析表明该基因不同于该抗原复合物先前克隆的成员，详细的血清学分析表明该基因可能编码85C成分。针对克隆TB1的表达产物产生的抗血清识别结核分枝杆菌提取物中的55 kDa蛋白。 55-kDa蛋白还具有纤连蛋白结合活性，与30 / 31-kDa家族一样，它是分枝杆菌病患者抗体应答的主要靶标。尽管这些克隆是通过使用相同的抗血清选择的，但通过血清学和DNA杂交进行的详细分析显示，它们代表了结核分枝杆菌表达的两种截然不同的纤连蛋白结合活性。结核分枝杆菌纤连蛋白结合抗原的进一步分析可能提供重要的见解，以了解其在介导与宿主免疫系统相互作用中的作用。

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