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首页> 外文期刊>Infection and immunity >Contribution of the pAD1-encoded cytolysin to the severity of experimental Enterococcus faecalis endophthalmitis.
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Contribution of the pAD1-encoded cytolysin to the severity of experimental Enterococcus faecalis endophthalmitis.

机译：pAD1编码的溶细胞素对实验性粪肠球菌眼内炎的严重程度的贡献。

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The contribution of the pAD1-encoded cytolysin to Enterococcus faecalis virulence in an experimental endophthalmitis model was studied by using isogenic strains differing only in the location of transposon Tn917. The course of experimental endophthalmitis in New Zealand White rabbits was evaluated by postoperative reduction in retinal neuroresponsiveness, thin-section histopathology, and transmission electron microscopy. Infections caused by cytolytic E. faecalis resulted in 99% loss of retinal function at postoperative day 3, while isogenic, noncytolytic strains produced reductions of only 74.2%. Light microscopy revealed near-total destruction of retinal architecture at 24 h postinfection with cytolytic E. faecalis, while noncytolytic strains produced few or no destructive changes. Transmission electron microscopy revealed tissue destruction in retinal layers as early as 6 h postinfection with cytolytic E. faecalis. In vivo and in vitro growth rates of cytolytic and noncytolytic E. faecalis showed similar kinetics. These data demonstrate the contribution of the pAD1-encoded cytolysin to both the course and the severity of experimental E. faecalis endophthalmitis.
机译：通过使用仅在转座子Tn917位置上不同的等基因菌株研究了pAD1编码的溶细胞素对实验性眼内炎模型中粪肠球菌毒力的贡献。新西兰白兔实验性眼内炎的病程是通过术后视网膜神经反应性降低，薄层组织病理学和透射电镜观察的。术后第3天，由溶细胞性大肠埃希菌引起的感染导致视网膜功能丧失99％，而同基因的非溶细胞性菌株仅造成74.2％的减少。光学显微镜显示，在溶细胞性粪肠球菌感染后24小时，视网膜结构几乎完全被破坏，而非溶细胞性菌株几乎没有破坏性变化。透射电子显微镜显示最早在溶细胞性粪肠球菌感染后6小时，视网膜层中的组织破坏。细胞溶解性和非细胞溶解性粪肠球菌的体内和体外生长速率显示相似的动力学。这些数据证明了pAD1编码的溶细胞素对实验性粪肠球菌眼内炎的进程和严重程度的贡献。

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