• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Intracellular growth of Afipia felis, a putative etiologic agent of cat scratch disease.
【24h】

Intracellular growth of Afipia felis, a putative etiologic agent of cat scratch disease.

机译：猫抓痕病的病原体阿非斯猫的细胞内生长。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The organism Afipia felis, which is though to be an etiologic agent of cat scratch disease, is a gram-negative rod that is clearly seen in infected tissue but is very difficult to isolate from clinical specimens; there has been only one report to date of the successful isolation and maintenance of the bacterium on artificial medium. We have found that A. felis will attach, invade via phagocytosis, and multiply intracellularly within the phagosomes of primary human monocytes and HeLa cells. Once in the cell, the bacterium appears to change morphologically, becoming longer and more pleomorphic, and loses its ability to grow on an artificial medium. Unique proteins have been identified in both the intra- and extracellular variants of A. felis. Convalescent-phase sera from patients with cat scratch disease react poorly with intracellular and extracellular bacteria, suggesting a poor humoral response. The tissue culture protocol presented has been used to isolate 14 new strains of A. felis and has for the first time permitted study of the pathogenesis of this unique organism.
机译：Afipia felis生物体虽然是猫抓病的病原体，但它是革兰氏阴性杆菌，在感染的组织中清晰可见，但很难从临床标本中分离出来。迄今为止，仅有一份关于在人工培养基上成功分离和维持细菌的报道。我们已经发现猫屎肠球菌将通过吞噬作用附着，侵入并在原代人单核细胞和HeLa细胞的吞噬体内在细胞内繁殖。一旦进入细胞，细菌就会出现形态变化，变得越来越长和多形，并失去在人工培养基上生长的能力。在猫屎曲霉的胞内和胞外变体中均已鉴定出独特的蛋白质。猫抓病患者的恢复期血清与细胞内和细胞外细菌的反应较差，表明体液反应较差。提出的组织培养方案已被用于分离14种新的猫曲霉菌株，并首次允许研究这种独特生物的发病机理。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号