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首页> 外文期刊>Infection and immunity >Impaired mucosal antibody response to cholera toxin in vitamin A-deficient rats immunized with oral cholera vaccine.
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Impaired mucosal antibody response to cholera toxin in vitamin A-deficient rats immunized with oral cholera vaccine.

机译：在口服霍乱疫苗免疫的维生素A缺乏型大鼠中，对霍乱毒素的粘膜抗体反应受损。

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To investigate the importance of vitamin A in the ability to respond to oral antigen administration, rats were fed a vitamin A-free diet. The animals were immunized perorally three times with a mixture of cholera toxin (CT) and a commercial cholera vaccine. The total immunoglobulin A (IgA) concentration as well as the specific IgA anti-CT antibody levels in serum and bile was significantly lower in the vitamin A-deficient animals than in the paired fed controls (animals that were fed a normal commercial diet in an amount equal to the amount the deficient animals consumed), while the levels of total and specific anti-CT IgG were not affected to the same extent by the vitamin A deficiency. The number of IgA anti-CT antibody-producing cells in the mesenteric lymph nodes after immunization was also significantly lower in the vitamin A-deficient rats than in the control rats. Supplementation of the diet with retinyl palmitate restored the ability to mount an IgA antibody response to the antigen, since the level of specific IgA anti-CT antibodies in relation to the total IgA concentration was as high in the vitamin A-supplemented group as in the paired fed control group. Restricted diet intake by itself did not affect the ability to respond adequately to the antigen since there was no difference in IgA anti-CT antibody level between paired fed rats and those being fed ad libitum. Assessment of transforming growth factor beta in cell cultures revealed no difference between vitamin A-deficient and paired fed animals. In summary, vitamin A deficiency resulted in a decreased number of IgA-producing cells, decreased IgA production, and a reduced ability to respond with IgA antibodies to the oral cholera vaccine.
机译：为了研究维生素A对口服抗原反应能力的重要性，给大鼠喂食了不含维生素A的饮食。用霍乱毒素（CT）和市售霍乱疫苗的混合物对动物进行三次口服免疫。缺乏维生素A的动物的血清和胆汁中总免疫球蛋白A（IgA）浓度以及特定的IgA抗CT抗体水平显着低于配对喂养的对照动物（以正常商业饮食喂养的动物）。含量等于缺乏动物的进食量），而总维生素C和特异性抗CT IgG的水平并未受到维生素A缺乏的相同程度的影响。缺乏维生素A的大鼠在免疫后肠系膜淋巴结中产生IgA抗CT抗体的细胞数量也明显低于对照组。在饮食中补充棕榈酸视黄酯可恢复针对抗原的IgA抗体反应的能力，因为在补充维生素A的人群中，相对于总IgA浓度而言，特定IgA抗CT抗体的水平相对于总IgA浓度高。配对对照组。饮食限制饮食本身并不影响对抗原充分反应的能力，因为成对喂养的大鼠和随意喂养的大鼠之间IgA抗CT抗体水平没有差异。对细胞培养物中转化生长因子β的评估表明，缺乏维生素A的动物和配对喂养的动物之间没有差异。总之，维生素A缺乏导致IgA产生细胞数量减少，IgA产生量降低以及对口腔霍乱疫苗的IgA抗体反应能力降低。

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