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首页> 外文期刊>Infection and immunity >Candidate vaccine antigens identified by antibodies from mice vaccinated with 15- or 50-kilorad-irradiated cercariae of Schistosoma mansoni.
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Candidate vaccine antigens identified by antibodies from mice vaccinated with 15- or 50-kilorad-irradiated cercariae of Schistosoma mansoni.

机译：通过用曼氏血吸虫的15或50公斤辐照的尾-接种疫苗的小鼠的抗体鉴定的候选疫苗抗原。

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In murine schistosomiasis, the highest levels of resistance to cercarial challenge are obtained by vaccination with radiation-attenuated cercariae. To identify candidate vaccine antigens relevant to the vaccine model, we examined parasite antigens recognized by antibodies from mice vaccinated with irradiated cercariae of Schistosoma mansoni. To optimize recognition of a wide spectrum of antigens, several factors that influence the level of protection in this model were varied; specifically, we examined the effect of (i) single versus multiple vaccinations with irradiated cercariae, (ii) the dose of irradiation (15 or 50 kilorads) administered to the cercariae, and (iii) the genetic background of mouse strains, high-responder (C57BL/6J) versus moderate-responder (CBA/J) mice. We found that the number of vaccinations did not alter antibody specificity but modified the relative antibody titers against particular antigens. The dose of irradiation used to attenuate the immunizing cercariae had a similar effect on antibody titers but in addition influenced antibody specificity. Only mice that had been vaccinated with moderately irradiated cercariae recognized cathepsin B (Sm31) and Sm32. Interestingly, when vaccinated mice of the two strains, C57BL/6J and CBA/J, were compared, differences in antibody responses to particular antigens were observed. Both strains recognized the integral membrane protein Sm23, glutathione S-transferase, and cathepsin B, whereas Sm32 and paramyosin were recognized only by CBA/J mice, and heat shock protein 70 was recognized exclusively by C57BL/6J mice. In this study, we conclusively identified six distinct antigens that are specifically recognized by the humoral immune response of vaccinated mice.
机译：在鼠血吸虫病中，通过对辐射衰减的尾c进行疫苗接种可获得最高水平的对子car虫病的抵抗力。为了鉴定与疫苗模型相关的候选疫苗抗原，我们检查了由曼氏血吸虫辐射尾接种疫苗的小鼠的抗体识别的寄生虫抗原。为了优化对广泛抗原的识别，影响该模型保护水平的几个因素有所不同。具体来说，我们研究了（i）辐照尾的单次接种与多次接种，（ii）尾cer给予的辐射剂量（15或50千拉德），以及（iii）小鼠品系的遗传背景，高响应者的效果（C57BL / 6J）与中度回应者（CBA / J）的老鼠。我们发现疫苗接种的数量并没有改变抗体的特异性，但是改变了针对特定抗原的相对抗体效价。用于减弱免疫尾cer的辐照剂量对抗体效价具有相似的作用，但另外影响抗体的特异性。只有已经接种了中度辐照尾cer的小鼠才能识别组织蛋白酶B（Sm31）和Sm32。有趣的是，当比较两种菌株的疫苗接种小鼠C57BL / 6J和CBA / J时，观察到了对特定抗原的抗体反应的差异。两种菌株均识别完整的膜蛋白Sm23，谷胱甘肽S-转移酶和组织蛋白酶B，而Sm32和副肌球蛋白仅被CBA / J小鼠识别，而热休克蛋白70仅被C57BL / 6J小鼠识别。在这项研究中，我们最终确定了六种不同的抗原，这些抗原被疫苗接种小鼠的体液免疫应答特异性识别。

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