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首页> 外文期刊>Infection and immunity >Chemotactic response to mucin by Serpulina hyodysenteriae and other porcine spirochetes: potential role in intestinal colonization.
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Chemotactic response to mucin by Serpulina hyodysenteriae and other porcine spirochetes: potential role in intestinal colonization.

机译：猪痢疾螺旋体和其他猪螺旋体对粘蛋白的趋化反应：在肠道定植中的潜在作用。

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Chemotaxis of porcine spirochetes towards a variety of mucins was measured quantitatively by a capillary method. A chemotaxis buffer consisting of 0.01 M potassium phosphate buffer (pH 7.0) and 0.2 mM L-cysteine hydrochloride was necessary for chemotaxis of spirochetes. The optimum incubation time and incubation temperature were 1 h and 40 degrees C, respectively. The mucin concentration also affected the chemotaxis observed, and a concentration of 1% (wt/vol) was near the optimum. Virulent Serpulina hyodysenteriae strains were chemotactic towards 1% (wt/vol) hog gastric mucin and 1% (wt/vol) porcine colonic mucin but not towards 1% (wt/vol) bovine submaxillary mucin. Virulent S. hyodysenteriae strains were significantly more chemotactic than avirulent strains of S. hyodysenteriae (SA3 and VS1), Serpulina intermedius, and Serpulina innocens. Other spirochetes belonging to the proposed group of spirochetes Anguillina coli were also not chemotactic. Pathogenicity of S. hyodysenteriae strains that cause swine dysentery may, in part, be attributed to their attraction to porcine intestinal mucus.
机译：用毛细管法定量测定猪螺旋体对多种粘蛋白的趋化性。由0.01 M磷酸钾缓冲液（pH 7.0）和0.2 mM L-半胱氨酸盐酸盐组成的趋化性缓冲液对于螺旋体的趋化性是必需的。最佳孵育时间和孵育温度分别为1 h和40摄氏度。粘蛋白浓度也影响所观察到的趋化性，并且1％（wt / vol）的浓度接近最佳。剧毒的猪痢疾短螺旋体菌株对1％（wt / vol）猪胃粘蛋白和1％（wt / vol）猪结肠粘蛋白具有趋化作用，但对1％（wt / vol）的牛上颌黏液则没有趋化作用。毒性猪痢疾链球菌菌株比猪痢疾链球菌（SA3和VS1），中间型Serpulina和无病性Serpulina innocens的无毒菌株明显更具趋化性。属于拟议的螺旋藻安圭拉菌群的其他螺旋藻也不趋化。引起猪痢疾的猪痢疾链球菌菌株的致病性可能部分归因于它们对猪肠粘液的吸引。

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