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首页> 外文期刊>Infection and immunity >Survival and replication of Rhodococcus equi in macrophages.
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Survival and replication of Rhodococcus equi in macrophages.

机译：马红球菌在巨噬细胞中的存活和复制。

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Rhodococcus equi is a facultative intracellular bacterium of macrophages that can cause serious pneumonia in both young horses and immunocompromised people. Essential to understanding rhodococcus pathogenesis is a quantitative documentation of the intracellular events that follow macrophage phagocytosis of the organism. By using a bacterial immunofluorescence staining assay, we verified the intracellular survival and replicative potential of R. equi in both murine peritoneal macrophages and equine alveolar macrophages in vitro. Following an initial lag period of 6 to 12 h, the intracellular numbers of R. equi begin to rise, often reaching macrophage-compromising levels by 48 h. A quantitative determination of bacterial growth by a novel image analysis cytometry technique confirmed our fluorescence microscopic results. By 48 h postinfection, bacterial numbers had increased by more than fivefold, and the majority of infected macrophages in the monolayer contained 10 or more bacteria per cell. The intracellular organisms were viable, as evidenced by the ability to incorporate radiolabeled uracil. The use of these techniques has identified differences in the in vitro replicative capacities of a virulent strain and an avirulent strain of R. equi. A clinical isolate of R. equi expressing a 17-kDa virulence-associated plasmid-encoded antigen was able to survive and replicate within macrophages, whereas an avirulent, non-plasmid-containing strain replicated poorly. These results suggest that plasmid-encoded bacterial virulence factors may contribute to the ability of R. equi to replicate within its host cell, the macrophage.
机译：马红球菌是巨噬细胞的兼性细胞内细菌，可在幼马和免疫功能低下的人中引起严重的肺炎。理解红球菌发病机理必不可少的是定量记录生物体巨噬细胞吞噬后的细胞内事件。通过使用细菌免疫荧光染色法，我们验证了鼠腹膜巨噬细胞和马肺泡巨噬细胞在体外R. equi的细胞内存活和复制潜力。在最初的6到12小时的滞后期之后，马齿。的细胞内数量开始增加，到48小时通常达到损害巨噬细胞的水平。通过新颖的图像分析细胞计数技术对细菌生长的定量测定证实了我们的荧光显微镜结果。到感染后48小时，细菌数量增加了五倍多，并且单层中大多数被感染的巨噬细胞每个细胞包含10个或更多细菌。细胞内生物体是可行的，如掺入放射性标记尿嘧啶的能力所证明。这些技术的使用已经确定了马力红球菌的毒力菌株和无毒力菌株的体外复制能力的差异。表达17-kDa毒力相关质粒编码抗原的马克斯菌的临床分离株能够在巨噬细胞中存活和复制，而无毒，不含质粒的菌株复制能力很差。这些结果表明，质粒编码的细菌毒力因子可能有助于马鞭毛虫在其宿主细胞巨噬细胞内复制的能力。

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