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首页> 外文期刊>Infection and immunity >Borrelia burgdorferi antigens that are targeted by antibody-dependent, complement-mediated killing in the rhesus monkey.
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Borrelia burgdorferi antigens that are targeted by antibody-dependent, complement-mediated killing in the rhesus monkey.

机译：恒河猴中抗体依赖性补体介导的杀伤性伯氏疏螺旋体抗原。

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We identified surface antigens of Borrelia burgdorferi that are targeted by antibody-dependent, complement-mediated killing (ADCK) in the rhesus monkey. For this purpose, we had available serum samples from three animals infected with B. burgdorferi JD1 by needle inoculation and from two monkeys that were infected with the same B. burgdorferi strain by Ixodes scapularis tick bite. Sera from monkeys from the first group contained antibodies to OspA and OspB detectable by Western blot (immunoblot) using whole B. burgdorferi antigens, whereas serum samples from animals in the second group did not. The targeting of OspA and OspB by functional antibodies was demonstrated directly by showing that ADCK was partially inhibited when antibodies were preincubated with an excess of soluble recombinant OspA or OspB. Simultaneous addition of OspA and OspB did not result in an additive inhibitory effect on ADCK, a result that suggests that the epitopes on OspA and that on OspB targeted by antibody in this mechanism are the same, or at least cross-reacting. The targeting of non-OspA, non-OspB surface antigens was inferred from the fact that sera from tick-inoculated animals, which did not contain detectable anti-OspA or anti-OspB antibodies, were able to effect ADCK. This killing effect was not inhibitable by the addition of recombinant OspA or OspB or both proteins together. We also showed that both immunoglobulin G and M antibodies participate in the ADCK mechanism in the rhesus monkey. Rhesus complement does not kill B. burgdorferi in vitro in the absence of antibody, and antibody alone is effective in killing only at serum dilutions lower than 1:15. However, such "complement-independent" antibodies were not present in all bleeds. Two main conclusions may be drawn from the analysis of our results. First, both OspA and OspB are targeted by the ADCK mechanism in the rhesus monkey. Second, one or more B. burgdorferi surface antigens that are neither OspA nor OspB also participate in ADCK.
机译：我们确定了恒河猴中抗体依赖性补体介导的杀伤（ADCK）靶向的伯氏疏螺旋体的表面抗原。为此，我们从三只动物通过针头接种感染了B. burgdorferi JD1动物，并从两只被肩tick突cap叮咬感染同一B. burgdorferi菌株的猴子获得了血清样品。第一组猴子的血清中含有使用完整的伯氏疏螺旋体抗原通过Western印迹（免疫印迹）检测到的OspA和OspB抗体，而第二组动物的血清样品则没有。通过显示当抗体与过量的可溶性重组OspA或OspB预温育时，ADCK被部分抑制，直接证明了功能性抗体对OspA和OspB的靶向作用。同时添加OspA和OspB不会导致对ADCK的累加抑制作用，这一结果表明，在这种机制下，OspA上的表位和抗体靶向的OspB上的表位相同，或者至少是交叉反应的。从不包含可检测的抗OspA或抗OspB抗体的壁虱接种动物的血清能够影响ADCK的事实推断出对非OspA，非OspB表面抗原的靶向。通过将重组OspA或OspB或两种蛋白质加在一起不能抑制这种杀伤作用。我们还显示免疫球蛋白G和M抗体均参与恒河猴的ADCK机制。在没有抗体的情况下，恒河猴补体不能在体外杀死B. burgdorferi，仅在低于1:15的血清稀释度下，单独的抗体才有效。但是，这种“不依赖补体的”抗体并不存在于所有出血中。从我们的结果分析中可以得出两个主要结论。首先，OspA和OspB都通过ADCK机制靶向恒河猴。第二，既不是OspA也不是OspB的一种或多种伯氏疏螺旋体表面抗原也参与ADCK。

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