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首页> 外文期刊>Infection and immunity >Sulfated glycoconjugate receptors for the Bordetella pertussis adhesin filamentous hemagglutinin (FHA) and mapping of the heparin-binding domain on FHA.
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Sulfated glycoconjugate receptors for the Bordetella pertussis adhesin filamentous hemagglutinin (FHA) and mapping of the heparin-binding domain on FHA.

机译：百日咳博德特氏菌粘附素丝状血凝素（FHA）的硫酸化糖缀合物受体，并在FHA上绘制肝素结合结构域。

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Filamentous hemagglutinin (FHA) is a major adhesin present on the surface of the gram-negative respiratory pathogen Bordetella pertussis. A number of binding mechanisms have been described for the interaction of FHA with eukaryotic cells. We have focused on its function as a sulfated polysaccharide-binding protein and on identifying potential receptors for FHA on the epithelial cell surface. Using a thin-layer overlay technique, we found that FHA binds specifically to sulfated glycolipids but not to gangliosides or other neutral glycolipids. These results suggest that epithelial cell surface sulfated glycolipids function as receptors for FHA. Further studies demonstrated that a Chinese hamster ovary (CHO) cell strain deficient in glycosaminoglycan expression exhibits greatly diminished attachment to FHA. By FHA-Affi-Gel chromatography, a putative receptor for FHA that has characteristics consistent with a heparan sulfate proteoglycan was isolated from epithelial cell extracts. In addition, by using recombinant FHA fusion proteins, a specific glycosaminoglycan-binding domain located near the N terminus of the FHA molecule was identified. Our results indicate that the B. pertussis adhesin FHA may utilize sulfated glycolipids and proteoglycans commonly found on the surface of human cells and tissues to initiate infection.
机译：丝状血凝素（FHA）是革兰氏阴性呼吸道病原性百日咳博德特氏菌表面上存在的主要粘附素。已经描述了许多用于FHA与真核细胞相互作用的结合机制。我们已经集中于其作为硫酸化多糖结合蛋白的功能，并致力于识别上皮细胞表面上FHA的潜在受体。使用薄层覆盖技术，我们发现FHA特异性结合硫酸化糖脂，但不结合神经节苷脂或其他中性糖脂。这些结果表明上皮细胞表面硫酸化的糖脂起FHA受体的作用。进一步的研究表明，缺乏糖胺聚糖表达的中国仓鼠卵巢（CHO）细胞株与FHA的附着力大大降低。通过FHA-Affi-Gel色谱法，从上皮细胞提取物中分离出具有与硫酸乙酰肝素蛋白聚糖相一致的特征的FHA推定受体。另外，通过使用重组FHA融合蛋白，鉴定了位于FHA分子的N末端附近的特异性糖胺聚糖结合结构域。我们的结果表明，百日咳博德特氏菌粘附素FHA可能利用通常在人细胞和组织表面上发现的硫酸化糖脂和蛋白聚糖来引发感染。

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