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首页> 外文期刊>Infection and immunity >Characterization of a novel Mycobacterium bovis secreted antigen containing PGLTS repeats.
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Characterization of a novel Mycobacterium bovis secreted antigen containing PGLTS repeats.

机译：新型牛分枝杆菌分泌的含PGLTS重复抗原的表征。

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Serum from naturally infected cattle was used to identify a novel Mycobacterium bovis antigen from an expression library. The first recombinant product identified was a fusion protein with lacZ (55 kDa). A clone containing the whole gene was also obtained. This clone expressed a 38-kDa protein. A rabbit serum against the recombinant antigen reacts in M. bovis supernatants with two proteins of 36 and 34 kDa. The new protein was called P36/P34. The gene cloned has a deduced amino acid sequence with a predicted molecular mass of 28 kDa, showing a characteristic signal sequence for exportation. The protein bears partial homology to a 28-kDa protein from M. leprae. An interesting feature of the P36/P34 sequence is that it contains several PGLTS repeats, which are not present in the M. leprae protein. Antigenic determinants seem also to be conserved between the two proteins because sera from leprosy patients recognized the recombinant M. bovis protein. The discrepancy among the molecular mass deduced from the sequence (28 kDa), that of the recombinant protein in Escherichia coli (38 kDa), and that of the native protein in M. bovis (36 and 34 kDa) could be attributed to posttranslational modifications or to the high proline content that may alter the migration properties of the protein. This antigen seems to be immunodominant during bovine tuberculosis, because 8 of 9 serum specimens from diseased cattle are reactive. The homology among the M. leprae 28-kDa protein, the protein described in this article, and a recently described M. tuberculosis protein suggests the existence of a new protein family in mycobacteria.
机译：来自自然感染牛的血清用于从表达文库中鉴定新型牛分枝杆菌抗原。鉴定出的第一个重组产物是与lacZ（55 kDa）的融合蛋白。还获得了包含整个基因的克隆。该克隆表达38-kDa蛋白。抗重组抗原的兔血清在牛分枝杆菌上清液中与两种36和34 kDa的蛋白质反应。这种新蛋白质称为P36 / P34。克隆的基因具有推定的氨基酸序列，预测的分子量为28 kDa，显示了用于输出的特征性信号序列。该蛋白与麻风分枝杆菌的28 kDa蛋白具有部分同源性。 P36 / P34序列的一个有趣特征是它包含几个PGLTS重复序列，而这些序列在麻风分枝杆菌蛋白中不存在。抗原决定簇似乎在这两种蛋白之间也是保守的，因为麻风病人的血清识别出重组牛分枝杆菌蛋白。由序列（28 kDa）推导的分子量，大肠杆菌中重组蛋白的分子量（38 kDa）和牛分枝杆菌中的天然蛋白（36和34 kDa）的分子量差异可能归因于翻译后修饰或脯氨酸含量高可能会改变蛋白质的迁移特性。该抗原似乎在牛结核病期间具有免疫优势，因为患病牛的9个血清标本中有8个具有反应性。麻风分枝杆菌28-kDa蛋白，本文所述蛋白和最近描述的结核分枝杆菌蛋白之间的同源性表明，在分枝杆菌中存在新的蛋白家族。

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