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首页> 外文期刊>Infection and immunity >Reduced expression of the functionally active complement receptor for iC3b but not for C3d on an avirulent mutant of Candida albicans.
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Reduced expression of the functionally active complement receptor for iC3b but not for C3d on an avirulent mutant of Candida albicans.

机译：在白色念珠菌的无毒突变体上，iC3b的功能性活性补体受体表达降低，但C3d的表达降低。

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Pseudohyphae of Candida albicans bear surface receptors for iC3b and C3d. In order to determine a possible role for these receptors in the pathogenesis of candidiasis, a spontaneous C. albicans mutant, m-10, which exhibits reduced ability to adhere in vitro to fibrin platelet clots and epithelial cells or to cause endocarditis in a rabbit model, and its parent wild-type (wt) strain were compared for receptor expression in rosetting assays with sheep erythrocytes carrying iC3b (EAC1423bi) or C3d (EAC1423d). An equally high attachment to wt and m-10 was seen with EAC1423d, whereas rosetting with EAC1423bi was reduced by 53% in m-10 compared with wt. In inhibition studies, rosetting of wt with EAC1423bi was markedly inhibited by culture filtrate, hyphal-cell extract, and DEAE-fractionated material prepared from wt (54, 87, and 70% decreases in rosetting, respectively), thus suggesting the presence of the soluble, functionally active iC3b receptor of C. albicans in each of these preparations. Minimal inhibition of iC3b rosetting, however, was seen with the identical materials from m-10 (21, 5, and 12%, respectively). All of the preparations from the two strains were equally effective in their inhibitory activities against rosetting of C3d. A human serum specimen obtained from a patient with chronic mucocutaneous candidiasis blocked iC3b rosetting of the wt strain almost completely. When used in an immunoblot, this serum recognized proteins of 68 to 71, 55, and 50 kilodaltons (kDa) in hyphal-cell extracts of the wt. With the same preparation of the avirulent mutant, only weak reactions with the 68- to 71-kDa and 55-kDa proteins occurred, while the 50-kDa protein was not detectable. Taken together, these results indicate that the expression of the functionally active iC3b receptor on C. albicans may be involved in the virulence of the organism, possibly by mediating adherence to mammalian cells.
机译：白色念珠菌的假菌丝带有iC3b和C3d的表面受体。为了确定这些受体在念珠菌病发病机理中的可能作用，是自发的白色念珠菌突变体m-10，其在兔模型中表现出降低的体外粘附于血纤蛋白血小板凝块和上皮细胞或引起心内膜炎的能力。在带有iC3b（EAC1423bi）或C3d（EAC1423d）的绵羊红细胞的玫瑰花试验中，比较了其野生亲本（wt）菌株及其受体的受体表达。用EAC1423d可以看到与wt和m-10的附着率相同，而与wt。相比，用eAC1423bi在m-10中的花环减少了53％。在抑制研究中，由EAC1423bi组成的wt的玫瑰花结被培养物滤液，菌丝提取物和由wt制成的DEAE分馏物显着抑制（玫瑰花结分别减少54％，87％和70％），因此表明这些制剂中的每一种均能溶解白色念珠菌的功能性活性iC3b受体。但是，使用m-10的相同材料（分别为21％，5％和12％）可以看到对iC3b玫瑰花的抑制作用最小。两种菌株的所有制剂对C3d玫瑰花结的抑制活性均相同。从患有慢性粘膜皮肤念珠菌病的患者获得的人血清标本几乎完全阻断了wt菌株的iC3b玫瑰花结。当用于免疫印迹时，该血清可识别野生型的菌丝细胞提取物中68至71、55和50道尔顿（kDa）的蛋白质。使用无毒突变体的相同制剂，仅发生与68-71-kDa和55-kDa蛋白的弱反应，而50-kDa蛋白则不可检测。综上所述，这些结果表明，在白色念珠菌上的功能性活性iC3b受体的表达可能与生物体的毒力有关，可能是通过介导对哺乳动物细胞的粘附来实现的。

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