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Gamma interferon-mediated inhibition of Eimeria vermiformis growth in cultured fibroblasts and epithelial cells.

机译:γ干扰素介导的培养的成纤维细胞和上皮细胞对艾美球虫生长的抑制。

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The growth of Eimeria vermiformis within cultured murine fibroblastlike (L-929) or rat epithelial-like (RATEC) cells was inhibited by treatment of the cells with the appropriate recombinant gamma interferon. The effect was apparent as a reduction in both the initial numbers of intracellular sporozoites and, to a much greater extent, the numbers of subsequent developmental stages. Pretreatment of the host cells was more effective than treatment in the early postinvasive period, and recombinant gamma interferon had no effect on the development of the parasite if added 24 h or later after the inoculation of sporozoites. Incubation of sporozoites in medium containing recombinant gamma interferon in no way affected their ability to invade or to grow within host cells. These findings indicate that the inhibitory effects of recombinant gamma interferon on the growth of E. vermiformis are mediated via the host cell and are directed mainly against the transforming sporozoite, although the ability of the sporozoite to invade the host cell was also reduced to some extent. The later developmental stages were refractory to the effects of this lymphokine.
机译:通过用适当的重组γ干扰素处理细胞,可以抑制培养的鼠成纤维细胞样(L-929)或大鼠上皮样细胞(RATEC)中的艾美尔球虫的生长。这种作用是明显的,既减少了细胞内子孢子的初始数目,又大大减少了后续发育阶段的数目。宿主细胞的预处理比入侵后早期的处理更有效,如果在接种子孢子后24小时或之后添加重组伽马干扰素,对寄生虫的生长没有影响。子孢子在含有重组γ干扰素的培养基中的培养决不影响它们侵入宿主细胞或在宿主细胞内生长的能力。这些发现表明重组γ-干扰素对蠕虫的生长的抑制作用是通过宿主细胞介导的,并且主要针对转化的子孢子,尽管子孢子侵入宿主细胞的能力也有所降低。 。后来的发展阶段对该淋巴因子的作用不利。

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