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首页> 外文期刊>Infection and immunity >Anaerobic growth of gonococci does not alter their Opa-mediated interactions with human neutrophils.
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Anaerobic growth of gonococci does not alter their Opa-mediated interactions with human neutrophils.

机译：淋球菌的厌氧生长不会改变其Opa介导的与人类嗜中性粒细胞的相互作用。

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摘要

Gonococci grown anaerobically (anaerobic gonococci) in the presence of nitrite induce the expression of at least three novel outer membrane proteins (PANs 1 to 3). Although PAN 1 is expressed by gonococci during gonorrhea, the function of the PAN proteins remains unknown. In the absence of serum, gonococci possessing opacity-associated (Opa, formerly PII) outer membrane proteins adhere to, stimulate, and are phagocytically killed by human neutrophils. Gonococci lacking Opa proteins demonstrate none of these activities. We investigated whether the PAN proteins, or any other characteristics of anaerobic gonococci, altered the ability of nonpiliated, Opa+ or Opa- gonococci to adhere to, stimulate, or be phagocytically killed by neutrophils. The expression of Opa4 by strain F62, as determined by its relative mobility on sodium dodecyl sulfate-polyacrylamide gels, appeared to be unaltered by anaerobic growth, as seen previously (V. L. Clark, L. A. Campbell, D. A. Palermo, T. M. Evans, and K. W. Klimpel, Infect Immun. 55:1359-1364, 1987). Anaerobic and aerobic Opa+ gonococci adhered to and stimulated neutrophils to the same extent. Similarly, anaerobic and aerobic Opa- gonococci adhered to and stimulated neutrophils equally poorly. Finally, anaerobic and aerobic Opa+ gonococci were equally sensitive to phagocytic killing by neutrophils, while anaerobic and aerobic Opa- gonococci were equally resistant to killing. Thus, the role of Opa proteins in mediating the interactions of gonococci with human neutrophils appears unaltered by anaerobic growth, and the PAN proteins, or other cryptic properties of anaerobic gonococci, do not seem to modulate or mediate these phenomena.
机译：在亚硝酸盐存在下厌氧生长的淋病球菌（厌氧性淋球菌）诱导至少三种新型外膜蛋白（PANs 1-3）的表达。尽管淋病期间淋球菌可表达PAN 1，但PAN蛋白的功能仍然未知。在没有血清的情况下，具有不透明度相关（Opa，以前称为PII）的淋球菌的外膜蛋白粘附，刺激并被人类嗜中性粒细胞吞噬。缺乏Opa蛋白的淋球菌没有显示出这些活性。我们调查了PAN蛋白或厌氧性淋球菌的任何其他特征是否改变了非毛细，Opa +或Opa-gonococci粘附，刺激或吞噬嗜中性粒细胞的能力。如先前所见（VL Clark，LA Campbell，DA Palermo，TM Evans和KW Klimpel，由F62菌株在十二烷基硫酸钠-聚丙烯酰胺凝胶上的相对迁移率确定），Opa4的表达似乎不受厌氧生长的影响。 55：1359-1364，1987）。厌氧和好氧的Opa +淋球菌对嗜中性粒细胞的附着和刺激程度相同。同样，厌氧和好氧卵球菌对嗜中性粒细胞的粘附和刺激同样差。最后，厌氧和好氧的Opa +淋球菌对嗜中性粒细胞的吞噬能力同样敏感，而厌氧和好氧的Opa +淋球菌对杀伤能力也同样敏感。因此，Opa蛋白在介导淋球菌与人类嗜中性粒细胞相互作用中的作用似乎不会因厌氧菌的生长而改变，并且PAN蛋白或厌氧菌的其他隐性特性似乎并未调节或介导这些现象。

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