...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Bacterial heat shock proteins directly induce cytokine mRNA and interleukin-1 secretion in macrophage cultures.
【24h】

Bacterial heat shock proteins directly induce cytokine mRNA and interleukin-1 secretion in macrophage cultures.

机译：细菌热激蛋白直接诱导巨噬细胞培养物中的细胞因子mRNA和白介素-1分泌。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacterial heat shock proteins (hsp) have been shown to be important immunogens stimulating both T cells and B cells. However, little is known concerning the direct interactions between hsp and macrophages. In this study, we demonstrated that treatment of macrophage cultures with purified bacterial hsp, including Legionella pneumophila hsp60, Escherichia coli GroEL, Mycobacterium tuberculosis hsp70, Mycobacterium leprae hsp65, and Mycobacterium bovis BCG hsp65, increased the steady-state levels of cytokine mRNA for interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-6, tumor necrosis factor alpha, and granulocyte-macrophage colony-stimulating factor as well as supernatant IL-1 secretion. This effect was shown not to be due to contamination of the hsp preparations with bacterial lipopolysaccharide. However, not all hsp induced cytokines; M. tuberculosis hsp10 showed minimal activity in our study. These results suggest that bacterial hsp might modulate immunity by rapidly and directly increasing cytokine production in macrophages.
机译：细菌热激蛋白（hsp）已被证明是刺激T细胞和B细胞的重要免疫原。但是，关于hsp和巨噬细胞之间的直接相互作用知之甚少。在这项研究中，我们证明了用纯化的细菌hsp处理巨噬细胞培养物，包括嗜肺军团杆菌hsp60，大肠杆菌GroEL，结核分枝杆菌hsp70，麻风分枝杆菌hsp65和牛分枝杆菌BCG hsp65，可增加白介素细胞因子mRNA的稳态水平-1 alpha（IL-1 alpha），IL-1 beta，IL-6，肿瘤坏死因子α和粒细胞巨噬细胞集落刺激因子以及上清液IL-1分泌。已显示该效果不是由于hsp制剂被细菌脂多糖污染所致。但是，并非所有的hsp都能诱导细胞因子。结核分枝杆菌hsp10在我们的研究中显示出最小的活性。这些结果表明细菌热休克蛋白可能通过迅速和直接增加巨噬细胞中细胞因子的产生来调节免疫力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号