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首页> 外文期刊>Infection and immunity >Self-transmissible R plasmids encoding CS31A among human Escherichia coli strains isolated from diarrheal stools.
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Self-transmissible R plasmids encoding CS31A among human Escherichia coli strains isolated from diarrheal stools.

机译：从腹泻大便中分离出的人大肠杆菌菌株中编码CS31A的自传递性R质粒。

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The CS31A antigen was first described for septicemic and enterotoxigenic bovine E. coli strains. In our study, of 597 human Escherichia coli strains isolated from diarrheagenic stools of hospitalized patients, 30 (5%) hybridized with the CS31A DNA probe. These CS31A-positive E. coli strains diffusely adhered to Caco-2 and/or HEp-2 cells and produced a major surface protein of either 30 or 30.5 kDa according to the strain. These proteins were antigenically related to the two forms of the CS31A antigen, namely, CS31A-L and CS31A-H. Genes encoding CS31A were located on 140-kb conjugative R plasmids. E. coli transconjugants expressed major surface proteins similar to those of the wild-type strains and adhered to Caco-2 and/or HEp-2 cells. An association of CS31A and another adhesive factor of the Dr family was found in 70% of wild-type strains, since 21 strains hybridized with the diffuse adhesion DNA probe corresponding to the accessory gene (daaC) of the F1845 adhesin. Comparison of the restriction patterns of the 140-kb R plasmids of the CS31A-positive E. coli strains showed these plasmids to be similar. Hybridization experiments indicated that the genes encoding CS31A and resistance to penicillin were located together on either of two 20- or 27-kb EcoRI restriction fragments in four E. coli strains. We reported a similar linkage between these genes in Klebsiella pneumoniae strains which produced CF29K, a CS31A-like antigen. These results suggest a horizontal transfer between E. coli and K. pneumoniae strains.
机译：首先描述了CS31A抗原用于败血性和肠毒素性牛大肠杆菌菌株。在我们的研究中，从住院患者的腹泻大便中分离出597株人类大肠杆菌菌株，其中30株（5％）与CS31A DNA探针杂交。这些CS31A阳性大肠杆菌菌株扩散粘附至Caco-2和/或HEp-2细胞，并根据菌株产生30或30.5 kDa的主要表面蛋白。这些蛋白质与CS31A抗原的两种形式即CS31A-L和CS31A-H抗原性相关。编码CS31A的基因位于140 kb的偶联R质粒上。大肠杆菌转结合蛋白表达的主要表面蛋白与野生型菌株相似，并粘附于Caco-2和/或HEp-2细胞。在70％的野生型菌株中发现了CS31A和Dr家族的另一个粘附因子的关联，因为21株菌株与对应于F1845粘附素辅助基因（daaC）的弥散性粘附DNA探针杂交。 CS31A阳性大肠杆菌菌株140 kb R质粒的限制性酶切图谱比较表明，这些质粒是相似的。杂交实验表明，编码CS31A和对青霉素的抗性的基因一起位于四个大肠杆​​菌菌株的两个20或27 kb EcoRI限制性片段中。我们报道了肺炎克雷伯氏菌菌株中的这些基因之间的相似联系，该菌株产生CF29K，一种CS31A样抗原。这些结果表明大肠杆菌和肺炎克雷伯氏菌菌株之间的水平转移。

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