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首页> 外文期刊>Infection and immunity >Haemophilus ducreyi attaches to and invades human epithelial cells in vitro.
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Haemophilus ducreyi attaches to and invades human epithelial cells in vitro.

机译：杜克氏嗜血杆菌在体外附着并侵袭人类上皮细胞。

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Haemophilus ducreyi is a sexually transmitted pathogen that causes genital ulcers and inguinal adenopathy. Because chancroidal ulcers are most commonly located on the foreskins of uncircumcised males, we utilized human foreskin epithelial cells (HFECs) to investigate the initial interaction of H. ducreyi with its host. The eight different strains of H. ducreyi that were studied varied in their abilities to attach to these epithelial cells, with six strains consistently attaching to > or = 90% of HFECs and two strains attaching to < 25% of HFECs. The strains with low levels of adherence also failed to exhibit chaining in broth culture and were avirulent in the rabbit model, suggesting that virulence in this model and attachment may be linked. The most adherent strain, LA228R, was further evaluated for its ability to invade HFECs and HEp-2 cells. Scanning electron microscopy and transmission electron microscopy of HFECs after interaction with LA228R produced images consistent with attachment, ingestion into vesicles, and escape from the vesicles into the cytoplasm. In addition, the gentamicin protection assay and inhibition of invasion by cytochalasin B and D indicated that LA228R was able to invade both HFECs and HEp-2 cells. Further examination of the mechanisms involved in the adherence and invasion of H. ducreyi into epithelial cells and their correlation with virulence will provide a better understanding of the pathogenesis of the disease caused by this important pathogen.
机译：杜克伊嗜血杆菌是一种性传播病原体，可导致生殖器溃疡和腹股沟淋巴结肿大。由于环状脉状溃疡最常位于未割包皮的男性的包皮上，因此我们利用人的包皮上皮细胞（HFEC）来研究杜克氏杆菌与其宿主的初始相互作用。研究的八种杜氏嗜血杆菌菌株附着于这些上皮细胞的能力各不相同，其中六种菌株始终附着于≥90％的HFEC，而另两种菌株附着于≤25％的HFEC。依从性低的菌株在肉汤培养中也没有表现出连锁作用，并且在兔模型中没有毒力，这表明该模型中的毒力和附着力可能是相关的。进一步评估了最贴壁的菌株LA228R侵袭HFEC和HEp-2细胞的能力。与LA228R相互作用后，HFEC的扫描电子显微镜和透射电子显微镜产生的图像与附着，摄入囊泡和从囊泡逃逸到细胞质一致。此外，庆大霉素的保护性测定以及细胞松弛素B和D对侵袭的抑制作用表明LA228R能够侵袭HFEC和HEp-2细胞。进一步研究杜氏嗜血杆菌粘附和侵袭上皮细胞的机制及其与毒力的相关性，将使人们更好地了解由这种重要病原体引起的疾病的发病机理。

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