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首页> 外文期刊>Infection and immunity >Targeting vaccinia virus-expressed secretory beta subunit of human chorionic gonadotropin to the cell surface induces antibodies.
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Targeting vaccinia virus-expressed secretory beta subunit of human chorionic gonadotropin to the cell surface induces antibodies.

机译：将牛痘病毒表达的人绒毛膜促性腺激素分泌型β亚基靶向细胞表面会诱导抗体。

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摘要

We carried out experiments designed to study the effect of a protein's localization on its immunogenicity. A novel cell-surface protein was generated from a small, glycosylated secretory protein. The DNA sequence encoding the entire precursor of the human chorionic gonadotropin beta (beta hCG) subunit was fused in the correct reading frame to the DNA sequence encoding the transmembrane and cytoplasmic domains of vesicular stomatitis virus glycoprotein. This chimeric gene was introduced into the vaccinia virus genome to generate a recombinant virus. The recombinant virus, when used to infect animal cells, expressed a 135-amino-acid beta hCG subunit anchored in cellular membranes by the 48 carboxy-terminal amino acids of vesicular stomatitis virus glycoprotein. The immunogenicity of this recombinant virus with respect to its ability to generate anti-hCG antibodies was compared with that of a second recombinant vaccinia virus expressing the native secretory form of beta hCG. All animals immunized with the vaccinia virus expressing beta hCG on the cell surface elicited high titers of anti-hCG antibodies. Even after a single immunization with the recombinant vaccinia virus, the anti-hCG antibody titers persisted for a long period of time (more than 6 months). None of the animals immunized with vaccinia virus expressing the native secretory form of beta hCG showed any hCG-specific antibody response.
机译：我们进行了旨在研究蛋白质定位对其免疫原性的影响的实验。从小的糖基化分泌蛋白产生了新的细胞表面蛋白。编码人绒毛膜促性腺激素β（βhCG）亚基整个前体的DNA序列在正确的阅读框中与编码水泡性口腔炎病毒糖蛋白的跨膜结构域和胞质结构域的DNA序列融合。将该嵌合基因引入痘苗病毒基因组中以产生重组病毒。当重组病毒用于感染动物细胞时，它表达了一个由水泡性口炎病毒糖蛋白的48个羧基末端氨基酸锚定在细胞膜上的135个氨基酸的βhCG亚基。将该重组病毒相对于其产生抗hCG抗体的能力的免疫原性与表达β-hCG天然分泌形式的第二种重组牛痘病毒的免疫原性进行了比较。用在细胞表面表达βhCG的牛痘病毒免疫的所有动物均引起高滴度的抗hCG抗体。即使用重组牛痘病毒单次免疫后，抗-hCG抗体滴度仍持续很长时间（超过6个月）。用表达天然分泌形式的βhCG的牛痘病毒免疫的动物均未显示任何hCG特异性抗体反应。

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