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首页> 外文期刊>Infection and immunity >Inhibition of human peripheral blood mononuclear cell proliferative response by glycosphingolipids from metacestodes of Echinococcus multilocularis.
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Inhibition of human peripheral blood mononuclear cell proliferative response by glycosphingolipids from metacestodes of Echinococcus multilocularis.

机译：多叶棘球oc球菌的糖鞘脂对人外周血单核细胞增殖反应的抑制作用。

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摘要

The effect on human peripheral blood mononuclear cells (PBMCs) of neutral glycosphingolipids extracted from metacestodes of the parasite Echinococcus multilocularis was investigated. Neutral glycosphingolipids inhibited [3H]thymidine uptake by human PBMCs upon stimulation by mitogens such as phytohemagglutinin A and pokeweed mitogen or by allogeneic Burkitt B cells. This effect was dose dependent and was related to a decrease in interleukin 2 (IL-2) synthesis, the expression of IL-2 receptors (CD25) being unmodified. Addition of exogenous recombinant IL-2 restored the cell proliferation. Partial inhibition of immunoglobulin G (IgG), IgA, and IgM synthesis was observed in the supernatant of cell culture in association with the inhibitory effect. Identification of active subfractions contained in the neutral glycosphingolipid fraction was also studied in relation to cell viability. The free ceramide fraction had an inhibitory effect, in part related to cell lysis, particularly at high concentration, while the monogalactosylceramides had a paradoxical effect: as an activator at low concentrations and as an inhibitor at high concentrations, with limited cell survival. The immunogenic neutral glycosphingolipids containing at least two carbohydrate residues, all having a structure based on Gal beta 1-->6Gal, were inhibitors of PBMC proliferation and showed good cell survival. These results suggest that parasite neutral glycosphingolipids may play an immunologically relevant role in alveolar hydatid disease.
机译：研究了从多虫棘球E虫的肠ces中提取的中性糖鞘脂对人外周血单核细胞（PBMC）的影响。中性糖鞘脂通过有丝分裂原（例如植物血凝素A和商陆有丝分裂原）或同种异体Burkitt B细胞刺激后，抑制人PBMC吸收[3H]胸苷。该作用是剂量依赖性的，并且与白介素2（IL-2）合成的减少有关，IL-2受体（CD25）的表达未改变。外源重组IL-2的添加恢复了细胞增殖。在细胞培养上清液中观察到免疫球蛋白G（IgG），IgA和IgM合成的部分抑制与抑制作用有关。还研究了与细胞活力相关的中性鞘糖脂级分中所含活性亚组分的鉴定。游离的神经酰胺级分具有抑制作用，部分与细胞裂解有关，特别是在高浓度下，而单半乳糖基神经酰胺具有悖论的作用：低浓度下为活化剂，高浓度下为抑制剂，细胞存活率有限。具有至少两个碳水化合物残基且均具有基于Gal beta 1-> 6Gal的结构的免疫原性中性鞘糖脂是PBMC增殖的抑制剂，并显示出良好的细胞存活率。这些结果表明，寄生虫中性鞘糖脂可能在肺泡包虫病中具有免疫学相关作用。

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