...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >DNA sequence analysis and restriction fragment length polymorphisms of the P1 gene of Haemophilus influenzae biogroup aegyptius associated with Brazilian purpuric fever.
【24h】

DNA sequence analysis and restriction fragment length polymorphisms of the P1 gene of Haemophilus influenzae biogroup aegyptius associated with Brazilian purpuric fever.

机译：与巴西紫癜热相关的流感嗜血杆菌生物群埃及埃及P1基因的DNA序列分析和限制性片段长度多态性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Brazilian purpuric fever (BPF) is a fulminant pediatric disease caused by specific strains of Haemophilus influenzae biogroup aegyptius. A conserved epitope on the P1 protein of strains of H. influenzae biogroup aegyptius is seen on most virulent isolates. The P1 protein from a Brazilian case-clone strain of H. influenzae biogroup aegyptius was analyzed by cloning and sequencing the gene. Three major variable regions are present within the P1 gene of the BPF clone in an architecture similar to that of the previously sequenced P1 genes from H. influenzae. The DNA sequence data of the P1 gene provided information for restriction fragment length polymorphism analyses among strains of H. influenzae biogroup aegyptius. Using PCR for amplification of the P1 gene, we found that AlwI restriction of this gene allowed for a highly accurate segregation of virulent strains of H. influenzae biogroup aegyptius associated with BPF. The strong association of virulent phenotypes with specific AlwI restriction patterns of the P1 gene provides a basis for the convenient and accurate identification of strains of H. influenzae biogroup aegyptius which cause BPF.
机译：巴西紫癜热（BPF）是一种由流感嗜血杆菌生物群埃及特有菌株引起的暴发性小儿疾病。在大多数强毒分离株中，流感嗜血杆菌埃及群生物群的菌株P1蛋白上存在一个保守的表位。通过克隆和测序该基因，分析了来自巴西流感嗜血杆菌生物群埃及个案克隆株的P1蛋白。 BPF克隆的P1基因中存在三个主要的可变区，其结构类似于先前从流感嗜血杆菌测序的P1基因。 P1基因的DNA序列数据为流感嗜血杆菌生物群埃及菌株之间的限制性片段长度多态性分析提供了信息。使用PCR扩增P1基因，我们发现该基因的AlwI限制允许高度精确地分离与BPF相关的流感嗜血杆菌生物群埃及毒株。毒力表型与P1基因的特定AlwI限制模式的强关联为方便准确地鉴定引起BPF的流感嗜血杆菌生物群埃及毒株提供了基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号