• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Characterization and immunoprotective properties of a monoclonal antibody against the major oocyst wall protein of Eimeria tenella.
【24h】

Characterization and immunoprotective properties of a monoclonal antibody against the major oocyst wall protein of Eimeria tenella.

机译：抗艾美球虫主要卵囊壁蛋白的单克隆抗体的表征和免疫保护特性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The oocyst wall of Eimeria spp. consists of a 10-nm-thick outer lipid layer and a 90-mm-thick inner layer of glycoprotein which has been described previously to be composed of a single major protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions and (125)I labelling of a oocyst wall fragments and of delipidated intact oocysts revealed a molecule of approximately 12 kDa as the major protein component of the oocyst wall of Eimeria tenella. An immunoglobulin M monoclonal antibody (c11B9F3) was produced against this 12-kDa oocyst wall protein sliced from a preparative SDS-polyacrylamide gel. Its reactivity by immunofluorescence against oocyst wall fragments and sporozoites or by immunoperoxidase assays of infected tissue sections was stage restricted to gametocytes and oocysts but pan-specific against all face of the oocyst wall. In chicks passively immunized with C11B9F3, oocyst output was significantly (P<0.01) reduced by 42 to 54% after homologous E. tenella infection and by 35% after heterologous Eimeria maxima infection compared with that of control groups. The results demonstrate the presence of a highly conserved, low-molecular-weight antigen on the oocyst wall and the gametocytes of Eimeria spp. which is a candidate for inclusion in a pan-specific, transmission-blocking vaccine against avian coccidiosis.
机译：艾美球虫的卵囊壁。由10nm厚的外脂层和90mm厚的糖蛋白内层组成，糖蛋白的内层先前已描述为由单一主要蛋白组成。十二烷基硫酸钠-聚丙烯酰胺钠凝胶在还原条件下的电泳以及卵囊壁碎片和已脱脂的完整卵囊的（125）I标记显示，大约12 kDa的分子是艾美叶球虫卵囊壁的主要蛋白质成分。针对从制备型SDS-聚丙烯酰胺凝胶切出的12kDa卵囊壁蛋白产生了免疫球蛋白M单克隆抗体（c11B9F3）。通过针对卵囊壁碎片和子孢子的免疫荧光或通过感染组织切片的免疫过氧化物酶测定，其反应性仅限于配子细胞和卵囊，但对卵囊壁的所有表面都具有泛特异性。与对照组相比，在用C11B9F3被动免疫的雏鸡中，同源大肠杆菌感染后，卵囊产量显着降低（P <0.01），降低了42％至54％，异源艾美球虫感染后降低了35％。结果表明，在艾美球虫属的卵囊壁和配子细胞上存在高度保守的低分子量抗原。可以作为针对禽球虫病的泛特异性，阻断传播的疫苗的候选药物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号