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首页> 外文期刊>Infection and immunity >The galactosyl-(alpha 1-4)-galactose-binding adhesin of Streptococcus suis: occurrence in strains of different hemagglutination activities and induction of opsonic antibodies.
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The galactosyl-(alpha 1-4)-galactose-binding adhesin of Streptococcus suis: occurrence in strains of different hemagglutination activities and induction of opsonic antibodies.

机译：猪链球菌的半乳糖基-（α1-4）-半乳糖结合粘附素：发生在不同血凝活性和调理抗体诱导的菌株中。

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摘要

The occurrence of the galactose-(alpha 1-4)-galactose-specific adhesin in Streptococcus suis, a pig and human pathogen causing sepsis, meningitis, and other serious infections, was studied. Poly- and monoclonal anti-bodies to the purified adhesin, as well as pigeon ovomucoid, a specific probe for the adhesin activity, detected one single protein band in extracts of S. suis. The adhesin was detected in all 23 strains studied, representing pathogenic serotypes (1, 2, 4, 5, 7, 8, and nontypeable) and including several weakly hemagglutinating or nonhemagglutinating strains and phase variants. The amount of adhesin detected was not correlated with the hemagglutination activity of the intact bacteria. Extraction of cells showing no binding of pigeon ovomucoid by ultrasonic treatment resulted in extracts with pigeon ovomucoid binding activity, suggesting that the adhesin was not accessible to the probe on the intact cells. Analysis of the amount of capsular polysaccharide revealed an inverse relationship between the hemagglutination activity and expression of capsular polysaccharide, thus suggesting a factor influencing adhesin accessibility. The purified adhesin was highly immunogenic and induced in preliminary experiments bactericidal activity in mice. Thus, the adhesin, with its specific binding mechanism to host cells and a proposed pathogenic role, is widely expressed among strains of different serotypes and therefore appears to represent a novel promising candidate for the development of a vaccine against S. suis.
机译：研究了猪链球菌，引起败血症，脑膜炎和其他严重感染的猪和人病原体中半乳糖-（α1-4）-半乳糖特异性粘附素的发生情况。纯化的粘附素的多抗体和单克隆抗体，以及粘附素活性的特异性探针鸽卵粘液，检测到猪链球菌提取物中的一条蛋白带。在所有研究的23个菌株中检测到粘附素，代表致病性血清型（1、2、4、5、7、8和不可分型），包括几个弱血凝或非血凝菌株和相变体。检测到的粘附素的量与完整细菌的血凝活性无关。通过超声处理显示不与鸽卵粘液结合的细胞提取物会产生具有鸽卵粘液结合活性的提取物，这表明完整细胞上的探针无法接触到粘​​附素。荚膜多糖含量的分析揭示了血凝活性和荚膜多糖表达之间的反比关系，因此暗示了影响粘附素可及性的因素。纯化的粘附素具有高度的免疫原性，并在初步实验中诱导了小鼠的杀菌活性。因此，具有与宿主细胞特异性结合机制和拟议的致病作用的粘附素在不同血清型的菌株中广泛表达，因此似乎代表了针对猪链球菌疫苗开发的新的有希望的候选者。

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