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首页> 外文期刊>Infection and immunity >Effect of Mild Acid Treatment on the Survival, Enteropathogenicity, and Protein Production in Vibrio parahaemolyticus
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Effect of Mild Acid Treatment on the Survival, Enteropathogenicity, and Protein Production in Vibrio parahaemolyticus

机译：温和酸处理对副溶血性弧菌存活，肠致病性和蛋白质产生的影响

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Vibrio parahaemolyticus is an important food-borne enteropathogen that encounters various adverse conditions in its native environment or during infection. Effects of mild acid treatment on survival under stress conditions, enteropathogenicity, and protein production in this pathogen were investigated. Logarithmically grown cells, at pH 7.5 shifted to pH 5.0 for 30 min, were more resistant to subsequent acid challenge at pH 4.4. A two-phase adaptive procedure (pH 5.8 for 30 min; pH 5.0 for 30 min) was better than a single-phase procedure for enhancing the acid tolerance of this pathogen. The acid-adapted cells were cross-protected against the challenges of low salinity and thermal inactivation. One-dimensional polyacrylamide gel electrophoresis revealed that proteins with molecular masses of 6.4, 9.0, 13.6, 16.3, 18.9, 22.9, 24.4, 28.3, 33.9, 36.9, 41.2, 47.6, 58.1, 65.6, 80.5, 88.2, and 96.9 kDa were induced or significantly enhanced, while proteins of 25.3, 30.1, 30.7, and 91.7 kDa were significantly inhibited. Two-dimensional polyacrylamide gel electrophoresis revealed that 20 species of proteins were induced or significantly enhanced, while 26 species were inhibited. In assays conducted using the suckling mouse model, enteropathogenicity of the acid-adapted cells was significantly enhanced in terms of intestine/body weight ratio and in vivo recovery of infected cells.
机译：副溶血性弧菌是一种重要的食源性肠病原菌，在其自然环境或感染过程中会遇到各种不利条件。研究了轻度酸处理对这种病原体在应激条件下存活，肠致病性和蛋白质产生的影响。在pH 7.5处对数生长的细胞移至pH 5.0 30分钟，对随后在pH 4.4处的酸攻击更具抵抗力。两阶段适应性程序（pH 5.8持续30分钟; pH 5.0持续30分钟）优于单相过程，以增强该病原体的耐酸性。酸适应的细胞被交叉保护，免受低盐度和热失活的挑战。一维聚丙烯酰胺凝胶电泳显示，诱导了分子质量为6.4、9.0、13.6、16.3、18.9、22.9、24.4、28.3、33.9、36.9、41.2、47.6、58.1、65.6、80.5、88.2和96.9 kDa的蛋白质。或显着增强，而25.3、30.1、30.7和91.7 kDa的蛋白质被显着抑制。二维聚丙烯酰胺凝胶电泳显示，诱导或显着增强了20种蛋白质，而抑制了26种。在使用乳鼠模型进行的测定中，酸适应性细胞的肠道致病性在肠/体重比和感染细胞的体内恢复方面得到了显着提高。

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