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首页> 外文期刊>Infection and immunity >A Globally Disseminated M1 Subclone of Group A Streptococci Differs from Other Subclones by 70 Kilobases of Prophage DNA and Capacity for High-Frequency Intracellular Invasion
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A Globally Disseminated M1 Subclone of Group A Streptococci Differs from Other Subclones by 70 Kilobases of Prophage DNA and Capacity for High-Frequency Intracellular Invasion

机译：A组链球菌的全球传播的M1亚克隆与其他亚克隆的噬菌体DNA的70千碱基和高频细胞内侵袭能力不同

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摘要

The M1inv+ subclone of M1 group A streptococci that spread globally in the late 1980s and early 1990s was previously identified by restriction fragment length polymorphism (RFLP), M protein, and SpeA exotoxin sequence analyses. Strains representing this subclone were characterized with regard to carriage of bacteriophage and capacity to invade cultured human epithelial cells. The M1inv+ subclone was found to harbor two entirely different prophages, phage T13 and phage T14, which together supplement its genome with nearly 70 kb of DNA. Phage T14 encodes the SpeA exotoxin and is closely related to the classic converting phage T12. Plaque-forming characteristics and RFLP analyses of phages T13 and T14 were compared to each other and to phage T12. Other subclones of M1, isolated in the 1970s to the early 1980s, lacked both prophages. The M1inv+ subclone was previously reported to be efficiently internalized by human epithelial cells. This potential was confirmed and expanded by comparing a variety of clinical isolates. The capacity for high-frequency invasion of epithelial cells was not transmitted to a laboratory strain of group A streptococci by the above-mentioned bacteriophages.
机译：以前通过限制性片段长度多态性（RFLP），M蛋白和SpeA外毒素序列分析确定了在1980年代末和1990年代初全球传播的M1群A链球菌的Minv +亚克隆。代表该亚克隆的菌株的特征在于噬菌体的运输和入侵培养的人上皮细胞的能力。发现M1inv +亚克隆带有两个完全不同的噬菌体，噬菌体T13和噬菌体T14，它们共同补充了其基因组近70 kb的DNA。噬菌体T14编码SpeA外毒素，并与经典的转化噬菌体T12密切相关。将噬菌体T13和T14的噬菌斑形成特性和RFLP分析相互比较，并与噬菌体T12进行比较。 M1的其他亚克隆在1970年代至1980年代初期分离，缺乏这两种建议。以前有报道称M1inv +亚克隆可被人上皮细胞有效内在化。通过比较各种临床分离株，证实并扩大了这种潜力。上皮细胞的高频侵袭能力没有通过上述噬菌体传递给A组链球菌的实验室菌株。

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