...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Characterization of the Importance of Polysaccharide Intercellular Adhesin/Hemagglutinin of Staphylococcus epidermidis in the Pathogenesis of Biomaterial-Based Infection in a Mouse Foreign Body Infection Model
【24h】

Characterization of the Importance of Polysaccharide Intercellular Adhesin/Hemagglutinin of Staphylococcus epidermidis in the Pathogenesis of Biomaterial-Based Infection in a Mouse Foreign Body Infection Model

机译：表皮葡萄球菌多糖细胞间粘附素/血凝素在小鼠异物感染模型中基于生物材料的感染的发病机理中的重要性的表征。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The production of biofilm is thought to be crucial in the pathogenesis of prosthetic-device infections caused byStaphylococcus epidermidis. An experimental animal model was used to assess the importance of biofilm production, which is mediated by polysaccharide intercellular adhesin/hemagglutinin (PIA/HA), in the pathogenesis of a biomaterial-based infection. Mice were inoculated along the length of a subcutaneously implanted intravenous catheter with either wild-type S. epidermidis1457 or its isogenic PIA/HA-negative mutant. The wild-type strain was significantly more likely to cause a subcutaneous abscess than the mutant strain (P < 0.01) and was significantly less likely to be eradicated from the inoculation site by host defense (P < 0.05). In addition, the wild-type strain was found to adhere to the implanted catheters more abundantly than the PIA/HA-negative mutant (P < 0.05). The reliability of the adherence assay was assessed by scanning electron microscopy. To exclude contamination or spontaneous infection, bacterial strains recovered from the experimental animals were compared to inoculation strains by analysis of restriction fragment length polymorphism patterns by pulsed-field gel electrophoresis. In vitro binding of the wild-type strain and its isogenic mutant to a fibronectin-coated surface was similar. These results confirm the importance of biofilm production, mediated by PIA/HA, in the pathogenesis of S. epidermidis experimental foreign body infection.
机译：生物膜的产生被认为在表皮葡萄球菌引起的假体装置感染的发病机理中至关重要。实验动物模型被用来评估生物膜产生的重要性，这种产生是由多糖细胞间粘附素/血凝素（PIA / HA）介导的，在基于生物材料的感染的发病机理中。沿野生型 S皮下植入静脉导管的长度接种小鼠。 epidermidis 1457或其同基因PIA / HA阴性突变体。与突变菌株相比，野生型菌株引起皮下脓肿的可能性更大（ P <0.01），并且通过宿主防御从接种部位清除的可能性也大大降低（ P <0.05）。此外，发现野生型菌株比PIA / HA阴性突变体（ P <0.05）更能粘附到植入的导管上。通过扫描电子显微镜评估粘附测定的可靠性。为了排除污染或自发感染，通过利用脉冲场凝胶电泳分析限制性片段长度多态性模式，将从实验动物中回收的细菌菌株与接种菌株进行比较。野生型菌株及其同基因突变体与纤连蛋白包被的表面的体外结合是相似的。这些结果证实了PIA / HA介导的生物膜生产在 S发病机理中的重要性。表皮动物实验性异物感染。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号