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首页> 外文期刊>Infection and immunity >Porcine Epithelial β-Defensin 1 Is Expressed in the Dorsal Tongue at Antimicrobial Concentrations
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Porcine Epithelial β-Defensin 1 Is Expressed in the Dorsal Tongue at Antimicrobial Concentrations

机译：猪上皮β-防御素1在抗菌浓度下在背舌中表达。

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Epithelial cells and phagocytes contain antimicrobial polypeptides that participate in innate host defense. A recently cloned porcine β-defensin, PBD-1, was detected by Northern organ blots exclusively in the tongue epithelium. We generated recombinant PBD-1 peptide by using a baculovirus-insect cell expression system and obtained two forms (PBD-142 and PBD-138), which differed by N-terminal truncation. Only PBD-142 was found in scrapings of the surface of the dorsal tongue or the buccal mucosa. Immunohistochemical staining with antibody to PBD-142revealed that PBD-1 was highly concentrated in an ～0.1-mm-thick layer in the cornified tips of the filiform (but not fungiform) papillae of the dorsal tongue and in the superficial squamous cell layers of the buccal mucosa. By scraping, extraction, and semiquantitative Western blotting, the concentration of PBD-1 in the dorsal tongue surface and the buccal mucosa was estimated at 20 to 100 μg/ml. PBD-1 had antibacterial activity against Escherichia coli,Salmonella typhimurium, Listeria monocytogenes, and Candida albicans in 10 mM sodium phosphate buffer (pH 7.4). Added NaCl progressively inhibited the activity of PBD-1 againstE. coli and C. albicans. In 10 mM sodium phosphate with 125 mM NaCl, the combinations of sublethal concentrations of PBD-1 and the porcine neutrophil peptide PG-3, PR-39, or PR-26 showed synergistic activity against E. coli or the multidrug-resistant S. typhimurium DT104. At its physiologic concentration, PBD-1 has antimicrobial effects under both low- and high-salt conditions encountered in the oral cavity and may contribute to the antimicrobial barrier properties of the dorsal tongue and oral epithelium.
机译：上皮细胞和吞噬细胞含有参与先天宿主防御的抗微生物多肽。通过北部器官印迹仅在舌头上皮中检测到了最近克隆的猪β-防御素PBD-1。我们使用杆状病毒-昆虫细胞表达系统产生了重组PBD-1肽，并获得了两种形式（PBD-1 42 和PBD-1 38 ），它们之间的差异为N。 -末端截断。在背舌或颊粘膜表面的刮擦中仅发现PBD-1 42 。用PBD-1 42 抗体进行的免疫组织化学染色显示，PBD-1高度浓缩在背舌丝状（但不是真菌状）乳头的角质化尖端〜0.1-mm厚的层中并且在颊粘膜的浅层鳞状细胞层中。通过刮取，提取和半定量蛋白质印迹，估计背舌表面和颊粘膜中PBD-1的浓度为20至100μg/ ml。 PBD-1在10 mM中对大肠杆菌，鼠伤寒沙门氏菌，李斯特菌李斯特菌和白色念珠菌具有抗菌活性磷酸钠缓冲液（pH 7.4）。添加的NaCl逐渐抑制了PBD-1针对em的活性。大肠菌和 C。白色的。在具有125 mM NaCl的10 mM磷酸钠中，亚致死浓度的PBD-1和猪嗜中性粒细胞肽PG-3，PR-39或PR-26的组合显示出对 E的协同活性。大肠杆菌或耐多药的 S。鼠伤寒 DT104。在其生理浓度下，PBD-1在口腔中遇到的低盐和高盐条件下均具有抗菌作用，并且可能有助于背舌和口腔上皮的抗菌屏障特性。

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