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首页> 外文期刊>Infection and immunity >Human Macrophage Gamma Interferon Decreases Gene Expression but Not Replication of Mycobacterium tuberculosis: Analysis of the Host-Pathogen Reciprocal Influence on Transcription in a Comparison of Strains H37Rv and CMT97
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Human Macrophage Gamma Interferon Decreases Gene Expression but Not Replication of Mycobacterium tuberculosis: Analysis of the Host-Pathogen Reciprocal Influence on Transcription in a Comparison of Strains H37Rv and CMT97

机译：人类巨噬细胞γ干扰素降低结核分枝杆菌的基因表达，但不能复制：宿主H病原体互作对转录的影响分析菌株H37Rv和CMT97的比较。

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Mycobacterium tuberculosis is an intracellular pathogen that readily survives and replicates in human macrophages (MΦ). Host cells have developed different mycobactericidal mechanisms, including the production of inflammatory cytokines. The aim of this study was to compare the MΦ response, in terms of cytokine gene expression, to infection with the M. tuberculosis laboratory strain H37Rv and the clinical M. tuberculosis isolate CMT97. Both strains induce the production of interleukin-12 (IL-12) and IL-16 at comparable levels. However, the clinical isolate induces a significantly higher and more prolonged MΦ activation, as shown by reverse transcription-PCR analysis of IL-1β, IL-6, IL-10, transforming growth factor beta, tumor necrosis factor alpha, and gamma interferon (IFN-γ) transcripts. Interestingly, when IFN-γ transcription is high, the number of M. tuberculosis genes expressed decreases and vice versa, whereas no mycobactericidal effect was observed in terms of bacterial growth. Expression of 11 genes was also studied in the two M. tuberculosis strains by infecting resting or activated MΦ and compared to bacterial intracellular survival. In both cases, a peculiar inverse correlation between expression of these genes and multiplication was observed. The number and type of genes expressed by the two strains differed significantly.
机译：结核分枝杆菌是一种细胞内病原体，很容易在人巨噬细胞（MΦ）中存活并复制。宿主细胞已发展出不同的分枝杆菌杀菌机制，包括炎性细胞因子的产生。这项研究的目的是比较细胞因子基因表达对Mem感染的MΦ反应。结核实验室菌株H37Rv和临床 M。结核病隔离株CMT97。两种菌株均诱导白介素12（IL-12）和IL-16的产生。然而，如IL-1β，IL-6，IL-10，转化生长因子β，肿瘤坏死因子α和γ干扰素的逆转录PCR分析所示，该临床分离株诱导了更高且更长的MΦ活化。 IFN-γ）转录物。有趣的是，当IFN-γ转录高时， M的数目。结核基因的表达减少，反之亦然，而在细菌生长方面未观察到分枝杆菌作用。在两个 M中还研究了11个基因的表达。通过感染静止或激活的MΦ感染结核菌菌株，并将其与细菌细胞内存活率进行比较。在这两种情况下，都观察到这些基因的表达与繁殖之间存在独特的逆相关性。两种菌株表达的基因的数量和类型明显不同。

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