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首页> 外文期刊>Infection and immunity >Salmonella enterica Serotype Typhimurium Elicits Cross-Immunity against a Salmonella enterica Serotype Enteritidis Strain Expressing LP Fimbriae from thelac Promoter
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Salmonella enterica Serotype Typhimurium Elicits Cross-Immunity against a Salmonella enterica Serotype Enteritidis Strain Expressing LP Fimbriae from thelac Promoter

机译：沙门氏菌血清型鼠伤寒沙门氏菌对表达来自lacac启动子的LP菌毛的沙门氏菌血清型肠炎沙门氏菌具有交叉免疫力

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The biological significance of fimbrial phase variation inSalmonella serotypes is currently unknown. Exposure to long polar (LP) fimbriae of Salmonella enterica serotype Typhimurium results in selection against lpf phase ON cells of serotype Enteritidis during a subsequent challenge, suggesting that fimbrial phase variation may be a mechanism to evade cross-immunity between Salmonella serotypes. This notion was tested by assessing the effect of an immune response against serotype Typhimurium LP fimbriae on colonization of mice with a serotype Enteritidis mutant in which the lpf promoter region was replaced with the Escherichia coli lac promoter. During a challenge with a serotype Enteritidis mutant carrying thelac promoter in front of the lpfoperon, significantly lower numbers were recovered from organs and feces of mice previously immunized with an lpf phase ON culture of serotype Typhimurium than from mice not previously exposed to LP fimbriae. Immunization with the lpf phase ON culture of serotype Typhimurium elicited antibodies that cross-reacted with a purified gluthathione-S-transferase–LpfA fusion protein of serotype Enteritidis. These data suggested that cross-immunity against LP fimbrial proteins cannot be evaded if phase variation on the transcriptional level is prevented by expressing thelpf operon from the lac promoter. These data hence support the idea that phase variation of LP fimbriae is a mechanism to evade cross-immunity between serotypes Enteritidis and Typhimurium.
机译：沙门氏菌血清型中纤维相变化的生物学意义目前未知。暴露于沙门氏菌血清型鼠伤寒菌的长极（LP）菌毛会导致在随后的攻击中针对肠炎沙门氏菌 lpf 期ON细胞的选择，这表明可能是纤维相的变化。一种逃避沙门氏菌血清型之间交叉免疫的机制。通过评估针对血清型鼠伤寒LP菌毛的免疫应答对血清型肠炎沙门氏菌突变体定植小鼠的效果进行了测试，在该小鼠中， lpf 启动子区域被 Escherichia coli lac < / em>启动子。在用 lpf 操纵子前面携带 lac 启动子的血清型肠炎沙门氏菌突变体进行攻击的过程中，从以前用免疫的小鼠的器官和粪便中回收的数量明显减少。鼠伤寒血清型的> lpf ON培养比以前未接触过LP菌毛的小鼠要好。血清型鼠伤寒的 lpf 阶段培养的免疫接种后，与纯化的血清型肠炎沙门氏菌- S -转移酶-LpfA融合蛋白发生交叉反应的抗体。这些数据表明，如果通过表达 lac 启动子的 lpf 操纵子来防止转录水平的相变，则无法避免针对LP纤维蛋白的交叉免疫。因此，这些数据支持以下观点：LP菌毛的相变是逃避血清型肠炎沙门氏菌和鼠伤寒沙门氏菌之间交叉免疫的机制。

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