A Recombinant Live Attenuated Strain of Vibrio cholerae Induces Immunity against Tetanus Toxin andBordetella pertussis Tracheal Colonization Factor

Inês Chen; Theresa M. Finn; Liu Yanqing; Qi Guoming; Rino Rappuoli; Mariagrazia Pizza

首页> 外文期刊>Infection and immunity >A Recombinant Live Attenuated Strain of Vibrio cholerae Induces Immunity against Tetanus Toxin andBordetella pertussis Tracheal Colonization Factor

【24h】

A Recombinant Live Attenuated Strain of Vibrio cholerae Induces Immunity against Tetanus Toxin andBordetella pertussis Tracheal Colonization Factor

机译：霍乱弧菌的重组减毒活株诱导针对破伤风毒素和百日咳博德特氏菌气管定居因子的免疫

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

An attenuated strain of Vibrio cholerae was used as a carrier for the expression of heterologous antigens such as fragment C from tetanus toxin (TetC) and tracheal colonization factor fromBordetella pertussis (Tcf). In vitro, high levels of protein were obtained when the Escherichia coli nirBpromoter was used and the bacteria were grown with low aeration. Intranasal immunization of mice with IEM101 expressing TetC elicited serum vibriocidal activity and induced antibodies against tetanus toxin which were protective against lethal challenge with 10 times the 50% lethal dose of tetanus toxin. Bacterial viability was essential for the induction of anti-TetC antibodies. Intranasal administration of IEM101 expressing Tcf induced a significant reduction in bacterial colonization of the tracheas of mice challenged with wild-type B. pertussis. These data are in agreement with the putative role of Tcf in Bordetellatracheal colonization. In conclusion, we have demonstrated thatV. cholerae may be used as a live vector to deliver heterologous antigens in vivo and that protection to both systemic and local challenge may be achieved.

机译：霍乱弧菌的减毒株用作表达异源抗原的载体，如破伤风毒素（TetC）的片段C和百日咳博德特氏菌（emf）的气管定植因子）。在体外，使用大肠杆菌nirB 启动子并在低通气条件下培养细菌，可以得到高水平的蛋白质。用表达TetC的IEM101进行小鼠的鼻内免疫可引起血清杀线活性，并诱导抗破伤风毒素的抗体，以50倍的破伤风毒素致死剂量的10倍可以致命。细菌生存力对于诱导抗TetC抗体至关重要。鼻内施用表达Tcf的IEM101诱导野生型 B攻击的小鼠气管细菌定植显着减少。百日咳。这些数据与Tcf在 Bordetella 气管定植中的假定作用相吻合。总之，我们证明了 V。霍乱可作为活载体在体内递送异源抗原，并可实现对全身和局部攻击的保护。 展开▼

著录项

来源
《Infection and immunity》 |1998年第4期|共6页

作者
Inês Chen; Theresa M. Finn; Liu Yanqing; Qi Guoming; Rino Rappuoli; Mariagrazia Pizza;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类医学免疫学;

关键词

相似文献

外文文献

中文文献

专利

1. Oral immunization with attenuated vaccine strains of Vibrio cholerae expressing a dodecapeptide repeat of the serine-rich Entamoeba histolytica protein fused to the cholera toxin B subunit induces systemic and mucosal antiamebic and anti-V. cholerae antibody responses in mice. [J] . E T Ryan, J R Butterton, T Zhang, Infection and immunity . 1997,第8期

机译：表达与融合霍乱毒素B亚基的富含丝氨酸的Entamoeba histolytica蛋白的十二肽重复序列的霍乱弧菌减毒疫苗株进行口服免疫，可诱导全身性和粘膜性抗贫血和抗V。小鼠的霍乱抗体反应。

2. Oral immunization with attenuated vaccine strains of Vibrio cholerae expressing a dodecapeptide repeat of the serine-rich Entamoeba histolytica protein fused to the cholera toxin B subunit induces systemic and mucosal antiamebic and anti-V. cholerae antibody responses in mice. [J] . E T Ryan, J R Butterton, T Zhang, Infection and immunity . 1997,第8期

机译：表达与融合霍乱毒素B亚基的富含丝氨酸的Entamoeba histolytica蛋白的十二肽重复序列的霍乱弧菌减毒疫苗株进行口服免疫，可诱导全身性和粘膜性抗贫血和抗V。小鼠的霍乱抗体反应。

3. Neutralizing antibodies and immunoprotection against pertussis and tetanus obtained by use of a recombinant pertussis toxin-tetanus toxin fusion protein. [J] . P Boucher, H Sato, Y Sato, Infection and immunity . 1994,第2期

机译：通过使用重组百日咳毒素-破伤风毒素融合蛋白获得的中和抗体和针对百日咳和破伤风的免疫保护。

4. Silencing of the Innate Immune Response by Self-Regulating Martx Toxins of Vibrio Cholerae and Vibrio Vulnificus [D] . ?Woida, Patrick James 2020

机译：通过自我调节的Vibrio Cholerae和Vibrio Wulnfificus的自我调节Martx毒素沉默

5. A Recombinant Live Attenuated Strain of Vibrio cholerae Induces Immunity against Tetanus Toxin and Bordetella pertussis Tracheal Colonization Factor [O] . Inês Chen, Theresa M. Finn, Liu Yanqing, 1998

机译：重组减毒霍乱弧菌活菌诱导针对破伤风毒素和百日咳博德特氏菌气管定居因子的免疫

6. Oral immunization with attenuated vaccine strains of Vibrio cholerae expressing a dodecapeptide repeat of the serine-rich Entamoeba histolytica protein fused to the cholera toxin B subunit induces systemic and mucosal antiamebic and anti-V. cholerae antibody responses in mice [O] . E T Ryan, J R Butterton, T Zhang, 1997

机译：用振动慢性慢性疫苗菌株的口服免疫，表达富含富含氧化肽的富含丝氨酸的肽肽蛋白融合给霍乱毒素B亚单位的蛋白质诱导全身和粘膜抗型和抗v。小鼠霍乱抗体反应

7. Isolation and Characterization of a Vibrio cholerae Strain Unable to Secrete Cholera Toxin, and Cloning and Characterization of Genes from Vibrio cholerae that Confer a Congo Red Dye Binding Phenotype on Escherichia coli. [R] . Coker, C. 1996

机译：无法分泌霍乱毒素的霍乱弧菌菌株的分离和鉴定，以及霍乱弧菌基因的克隆和表征，该基因在大肠杆菌中具有刚果红染料结合表型。

1. 一株减毒百日咳鲍特菌的构建及生物学特性分析 [J] . 田苗苗 ,张优仪 ,王小莲 . 微生物与感染 . 2018,第001期

2. 淋球菌重组与减毒鼠伤寒沙门氏菌活疫苗口服免疫的初步研究 [J] . 吴卫 ,谢匡成 . 上海免疫学杂志 . 1998,第002期

3. 低氧诱导因子-1α和角质细胞生长因子双基因重组减毒沙门菌菌株的构建及其在肠上皮细胞中的表达 [J] . 哈小琴 ,李晓云 ,邓芝云 . 解放军医药杂志 . 2013,第011期

4. 低氧诱导因子-1α和角质细胞生长因子双基因重组减毒沙门菌菌株的构建及其在肠上皮细胞中的表达 [J] . 哈小琴 ,李晓云 ,邓芝云 . 解放军医药杂志 . 2013,第011期

5. 肠毒素源性定居因子CS6在减素伤寒沙门氏菌中表达及免疫原性的研究 [J] . 滕家波 ,张毅 . 微生物学报 . 1999,第006期

6. 减毒鸡白痢沙门氏菌?crpC79-13株的构建及其作为活疫苗对雏鸡免疫活性初步观察 [C] . 郁川 ,程相朝 ,赵战勤 . 中国畜牧兽医学会动物微生态学分会第四届第十次全国学术研讨会暨动物微生态企业发展论坛 . 2010

7. TGEV S基因减毒鼠伤寒沙门氏菌重组活载体疫苗株的初步构建 [A] . 秦学远 . 2008

1. 百日咳博德特氏菌和副百日咳博德特氏菌的特异性检测用引物探针组合和试剂盒 [P] . 中国专利： CN102634596B . 2016.08.03

2. 百日咳博德特氏菌和副百日咳博德特氏菌的特异性检测用引物探针组合和试剂盒 [P] . 中国专利： CN102634596A . 2012-08-15

3. The recombinant plasmid expressing the cloned gene CEF (CHO CELL ELONGATING FACTOR) VIBRIO CHOLERAE (OPTIONS) AND STRAIN ESCHERICHIA COLI-PRODUCER CEF (CHO CELL ELONGATING FACTOR) VIBRIO CHOLERAE (OPTIONS) [P] . 外国专利： RU2005115264A . 2006-11-20

机译：表达克隆基因CEF（CHO细胞长效因子）霍乱弧菌（OPTIONS）和应变埃希氏菌大肠菌CEF（CHO细胞长效因子）VIBRIO CHOLERAE（OPTIONS）的重组质粒

4. RECOMBINANT PLASMIDE DNA P1EM3 ENCODING SYNTHESIS OF B-SUBUNIT OF CHOLERA TOXIN, METHOD OF ITS CONSTRUCTING, AND STRAIN OF VIBRIO CHOLERAE BACTERIA PRODUCING B-SUBUNIT OF CHOLERA TOXIN [P] . 外国专利： SU1505022A1 . 1991-10-15

机译：编码霍乱毒素B亚基的重组质粒DNA P1EM3的合成，构建方法及产霍乱弧菌B-亚基的霍乱弧菌细菌的生产

5. STRAIN OF BACTERIUM VIBRIO CHOLERAE KM-168 AS BASE FOR CONSTRUCTION OF LIVE VACCINE AGAINST DIARRHEA DISEASE CAUSED BY PATHOGENIC STRAINS OF VIBRIO CHOLERAE O139 [P] . 外国专利： RU2236455C1 . 2004-09-20

机译：霍乱弧菌细菌KM-168作为构建活疫苗的基础，以抵抗霍乱弧菌O139的致病性菌株引起的腹泻

相关主题

A Recombinant Live Attenuated Strain of Vibrio cholerae Induces Immunity against Tetanus Toxin andBordetella pertussis Tracheal Colonization Factor

摘要

著录项

相似文献

相关主题

期刊订阅