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首页> 外文期刊>Infection and immunity >The 102-Kilobase pgm Locus of Yersinia pestis: Sequence Analysis and Comparison of Selected Regions among Different Yersinia pestis and Yersinia pseudotuberculosis Strains
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The 102-Kilobase pgm Locus of Yersinia pestis: Sequence Analysis and Comparison of Selected Regions among Different Yersinia pestis and Yersinia pseudotuberculosis Strains

机译：鼠疫耶尔森菌的102千碱基pgm位点：鼠疫耶尔森菌和假结核耶尔森菌菌株之间选定区域的序列分析和比较

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We report the complete 119,443-bp sequence of the pgmlocus from Yersinia pestis and its flanking regions. Sequence analysis confirms that the 102-kb unstable pgmlocus is composed of two distinct parts: the pigmentation segment and a high-pathogenicity island (HPI) which carries virulence genes involved in iron acquisition (yersiniabactin biosynthetic gene cluster). Within the HPI, three genes coding for proteins related to phage proteins were uncovered. They are located at both extremities indicating that the entire HPI was acquired en bloc by phage-mediated horizontal transfer. We identified, within the pigmentation segment, two novel loci that may be involved in virulence: a fimbriae gene cluster and a locus probably encoding a two component regulatory system similar to the BvgAS regulatory system of Bordetella pertussis. Three genes containing frameshift mutations and two genes interrupted by insertion element insertion were found within this region. To investigate diversity among different Y. pestis and Yersinia pseudotuberculosis strains, the sequence of selected regions of the pgm locus and flanking regions were compared from 20 different Y. pestis and 10 Y. pseudotuberculosis strains. The results showed that the genes interrupted in Y. pestis are intact in Y. pseudotuberculosis. However, one of these mutations, in thebvgS homologue, is only present in Y. pestisstrains of biovar Orientalis and not in those of the biovars Antiqua and Medievalis. The results obtained by analysis of variable positions in the sequence are in accordance with historical records, confirming that biovar Orientalis is the most recent lineage. Furthermore, sequence comparisons among 29 Yersinia strains suggest thatY. pestis is a recently emerged pathogen that is probably entering the initial phase of reductive evolution.
机译：我们报告了鼠疫耶尔森菌及其侧翼区域的 pgm 基因座的完整119,443-bp序列。序列分析证实了102kb不稳定的 pgm 基因座由两个不同部分组成：色素沉着部分和一个高致病性岛（HPI），该岛携带与铁获得有关的毒力基因（耶尔西菌素生物合成基因簇） ）。在HPI中，发现了三个编码与噬菌体蛋白相关的蛋白的基因。它们位于两个末端，表明整个HPI是通过噬菌体介导的水平转移整体获得的。我们在色素沉着区中确定了两个可能与毒力有关的新基因座：一个菌毛基因簇和一个可能编码类似于百日咳博德特氏菌的BvgAS调节系统的两组分调节系统的基因座。在该区域内发现了三个含有移码突变的基因和两个被插入元件插入打断的基因。调查不同 Y之间的多样性。通过对20个不同的 Y菌株中的鼠疫和假单胞菌耶尔森氏菌菌株，分别选择了 pgm 基因座的选定区域和侧翼区域的序列。鼠疫和10 Y。假结核菌株。结果表明，基因在 Y处被打断。瘟疫在 Y中完整无缺。假结核。然而，在 bvgS 同源物中的这些突变之一仅在 Y中存在。东方生物变种的鼠疫菌株，而不是Antiqua和Medievalis生物变种的鼠疫菌株。通过分析序列中的可变位置获得的结果与历史记录一致，证实了东方生物是最新的血统。此外，在29株耶尔森氏菌中的序列比较表明 Y。瘟病是一种新近出现的病原体，可能正进入还原性进化的初始阶段。

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