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首页> 外文期刊>Infection and immunity >Involvement of Mitogen-Activated Protein Kinase Pathways in Interleukin-8 Production by Human Monocytes and Polymorphonuclear Cells Stimulated with Lipopolysaccharide or Mycoplasma fermentans Membrane Lipoproteins
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Involvement of Mitogen-Activated Protein Kinase Pathways in Interleukin-8 Production by Human Monocytes and Polymorphonuclear Cells Stimulated with Lipopolysaccharide or Mycoplasma fermentans Membrane Lipoproteins

机译：丝裂素活化的蛋白激酶途径参与人单核细胞和脂多糖或发酵支原体膜脂蛋白刺激的多形核细胞白细胞介素8的生产。

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摘要

Interleukin-8 (IL-8) is a chemokine that belongs to the α-chemokine or CXC subfamily and is produced by a wide variety of human cells, including monocytes and polymorphonuclear cells (PMN). IL-8 is secreted in response to inflammatory stimuli, notably bacterial products such as lipopolysaccharide (LPS), but little is known about the mechanisms by which these agents mediate IL-8 induction. In this report, we show that Mycoplasma fermentans lipid-associated membrane proteins (LAMPf) induce the production of high levels of IL-8 by THP-1 (human monocyte) cells and PMN at the same extent as LPS. It was previously demonstrated that stimulation of monocytic cells with either LPS or LAMPf led to a series of common downstream signaling events, including the activation of protein tyrosine kinase and of mitogen-activated protein kinase cascades. By using PD-98059 and SB203580, two potent and selective inhibitors of MEK1 (a kinase upstream of ERK1/2) and p38, respectively, we have demonstrated that both ERK1/2 and p38 cascades play a key role in the production of IL-8 by monocytes and PMN stimulated with bacterial fractions.
机译：白介素8（IL-8）是一种趋化因子，属于α趋化因子或CXC亚家族，由多种人类细胞产生，包括单核细胞和多形核细胞（PMN）。 IL-8是响应炎症刺激而分泌的，尤其是细菌产物，例如脂多糖（LPS），但对于这些试剂介导IL-8诱导的机制知之甚少。在本报告中，我们显示了发酵支原体脂质相关膜蛋白（LAMPf）诱导THP-1（人类单核细胞）细胞和PMN产生高水平的IL-8，其程度与LPS。先前已证明，用LPS或LAMPf刺激单核细胞会导致一系列常见的下游信号转导事件，包括蛋白酪氨酸激酶和丝裂原活化蛋白激酶级联反应的激活。通过使用PD-98059和SB203580（分别是MEK1（ERK1 / 2上游的激酶）和p38的两种有效和选择性抑制剂），我们已经证明ERK1 / 2和p38级联在IL-产生中起关键作用8由单核细胞和PMN细菌级分刺激。

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