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首页> 外文期刊>Infection and immunity >Towards a Taenia solium Cysticercosis Vaccine: an Epitope Shared by Taenia crassiceps and Taenia solium Protects Mice against Experimental Cysticercosis
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Towards a Taenia solium Cysticercosis Vaccine: an Epitope Shared by Taenia crassiceps and Taenia solium Protects Mice against Experimental Cysticercosis

机译：牛带en虫囊病的疫苗：牛带en虫和牛带en虫共享的一个抗原决定簇保护小鼠免受实验性s虫病的侵害。

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The Taenia crassiceps recombinant antigen KETc7 has been shown to be effective as a vaccine against experimental murine cysticercosis, a laboratory model used to test potentially promising molecules against porcine Taenia soliumcysticercosis. Based on the deduced amino acid sequence of this proline-rich polypeptide, three fragments, GK-1, GK-2, and GK-3, were chemically synthesized in linear form. Of the three peptides, only GK-1 induced sterile protection against T. crassicepscysticercosis in 40 to 70% of BALB/cAnN male mice. GK-1 is an 18-amino-acid peptide which contains at least one B-cell epitope, as demonstrated by its ability to induce an antibody response to the peptide and T. crassiceps antigen without need of a carrier protein. Immunofluorescence studies revealed that anti-GK1 antibodies strongly react with the native protein in the tegument ofT. crassiceps and also with anatomical structures ofT. solium eggs, oncospheres, cysticercus, and tapeworm. GK-1 also contains at least one T-cell epitope, capable of stimulating the proliferation of CD8+ and to a lower extent CD4+ T cells primed either with the free peptide orT. crassiceps total antigen. The supernatant of the stimulated cells contained high levels of gamma interferon and low levels of interleukin-4. Similar results were obtained with T cells tested for intracellular cytokine production, an indication of the peptide’s capacity to induce an inflammatory response. The remarkable protection induced by GK-1 immunization, its physicochemical properties, and its presence in all developmental stages ofT. solium point to this synthetic peptide as a strong candidate in the construction of a synthetic vaccine againstT. solium pig cysticercosis.
机译：已经显示出 Taenia crassiceps 重组抗原KETc7作为抗鼠型鼠囊尾rc病的疫苗是有效的，这是一种实验室模型，用于测试潜在的有前景的分子对抗猪<虫Ta囊尾rc病。基于该富含脯氨酸的多肽的推导的氨基酸序列，化学合成了线性形式的三个片段GK-1，GK-2和GK-3。在这三种肽中，只有GK-1诱导针对 T的无菌保护。在40％至70％的BALB / cAnN雄性小鼠中出现了囊尾eps囊虫病。 GK-1是一种18个氨基酸的肽，包含至少一个B细胞表位，如其诱导该肽和 T抗体反应的能力所证明。不需要载体蛋白的猪ass抗原。免疫荧光研究表明，抗GK1抗体与天然蛋白强烈融合，形成了Tem的皮层。并具有 T的解剖结构。 lium虫卵，癌球，囊虫和tape虫。 GK-1还包含至少一个T细胞表位，能够刺激CD8 + 的增殖，并在较低程度上刺激用游离肽引发的CD4 + T细胞或 T。毛刺总抗原。刺激细胞的上清液含有高水平的γ干扰素和低水平的白介素4。测试细胞内细胞因子产生的T细胞获得了相似的结果，表明该肽诱导炎症反应的能力。 GK-1免疫诱导的显着保护作用，其理化性质以及在Tem的所有发育阶段均存在。 solium 指出该合成肽是构建抗 T合成疫苗的强力候选者。猪lium虫病。

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