A Lithium Chloride-Extracted, Broad-Spectrum-Adhesive 42-Kilodalton Protein ofStaphylococcus epidermidis Is Ornithine Carbamoyltransferase

Muzaffar Hussain; Georg Peters; Gursharan S. Chhatwal; Mathias Herrmann

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A Lithium Chloride-Extracted, Broad-Spectrum-Adhesive 42-Kilodalton Protein ofStaphylococcus epidermidis Is Ornithine Carbamoyltransferase

机译：表皮葡萄球菌的氯化锂提取，广谱粘附42 Kilodalton蛋白是鸟氨酸氨基甲酰转移酶。

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To identify novel putative staphylococcal adhesins, lithium chloride extraction (an established method for selective surface molecule solubilization) was employed. N-terminal sequencing and functional assays identified a 42-kDa fibronectin-binding protein fromStaphylococcus epidermidis as ornithine carbamoyltransferase (OCTase). However, OCTase was not recognizable extracellularly, and this fact together with the fact that LiCl induced DNA release and a decrease in viability suggests that LiCl extraction may not be the method of choice for selective surface molecule extraction from staphylococci.

机译：为了鉴定新型推定的葡萄球菌粘附素，采用了氯化锂萃取（一种建立的选择性表面分子增溶方法）。 N-末端测序和功能分析确定了表皮葡萄球菌的42 kDa纤连蛋白结合蛋白为鸟氨酸氨基甲酰转移酶（OCTase）。但是，OCTase在细胞外无法识别，这一事实以及LiCl诱导DNA释放和活力降低的事实表明，LiCl提取可能不是从葡萄球菌中选择性提取表面分子的选择方法。

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《Infection and immunity》 |1999年第12期|共3页
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Muzaffar Hussain; Georg Peters; Gursharan S. Chhatwal; Mathias Herrmann;
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中图分类医学免疫学;
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1. Activity of the liver enzyme ornithine carbamoyltransferase (OTC) in blood: LC-MS/MS assay for non-invasive diagnosis of ornithine carbamoyltransferase deficiency [J] . Krijt Jakub, Sokolova Jitka, Jesina Pavel, Clinical chemistry and laboratory medicine: CCLM . 2017,第8期

机译：血液中肝酶的活性血液中的血液：LC-MS / MS测定用于非侵入性诊断的鸟氨酸氨基甲酰基转移酶缺乏
2. A Fibrinogen-Binding Protein ofStaphylococcus epidermidis [J] . Martin Nilsson, Lars Frykberg, Jan-Ingmar Flock, Infection and immunity . 1998,第6期

机译：表皮葡萄球菌的纤维蛋白原结合蛋白
3. The clinically variable R40H mutant ornithine carbamoyltransferase shows cytosolic degradation of the precursor protein in CHO cells. [J] . Mavinakere M, Morizono H, Shi D, Journal of inherited metabolic disease . 2001,第6期

机译：临床上可变的R40H突变型鸟氨酸氨基甲酰基转移酶显示出CHO细胞中前体蛋白的胞质降解。
4. ADHESION OF 3T3 FIBROBLASTS AND STAPHYLOCOCCUS EPIDERMIDIS TO SURFACES MODIFIED WITH EXTRACELLULAR MATRIX PROTEINS [C] . S Dexter, M Camara, M Davies, International symposium on controlled release of bioactive materials;Consumer diversified products conference . 2001

机译：将3T3成纤维细胞和表皮葡萄球菌粘附至修饰的细胞外基质蛋白表面
5. Mechanisms of Staphylococcus epidermidis RP62A adhesion to biomedical polymers: The role of platelets, plasma proteins and polymer surface characteristics as studied under dynamic flow. [D] . Wang, I-Wen. 1996

机译：表皮葡萄球菌RP62A粘附于生物医学聚合物的机理：在动态流动下研究的血小板，血浆蛋白和聚合物表面特征的作用。
6. Note: A Lithium Chloride-ExtractedBroad-Spectrum-Adhesive 42-Kilodalton Protein ofStaphylococcus epidermidis Is OrnithineCarbamoyltransferase [O] . Muzaffar Hussain, Georg Peters, Gursharan S. Chhatwal, 1999

机译：注意：氯化锂萃取的广谱粘附性42-千达尔顿蛋白表皮葡萄球菌是鸟氨酸氨基甲酰转移酶
7. Primary and quaternary structure of the catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa Extensive sequence homology with the anabolic ornithine carbamoyltransferases of Escherichia coli [O] . Baur, Heinz H., LUETHI, Ernst E., Haas, Didier, 1987

机译：铜绿假单胞菌分解代谢鸟氨酸氨基甲酰基转移酶的一级和四级结构与大肠杆菌的合成代谢鸟氨酸氨基甲酰基转移酶的广泛序列同源性

A Lithium Chloride-Extracted, Broad-Spectrum-Adhesive 42-Kilodalton Protein ofStaphylococcus epidermidis Is Ornithine Carbamoyltransferase

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