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首页> 外文期刊>Infection and immunity >Acute Clinical Disease in Cats following Infection with a Pathogenic Strain of Bartonella henselae(LSU16)
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Acute Clinical Disease in Cats following Infection with a Pathogenic Strain of Bartonella henselae(LSU16)

机译：感染汉氏巴尔通体（LSU16）致病菌后猫的急性临床疾病

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Bartonella henselae is the causative agent of human cat scratch disease as well as several serious sequelae of infections, including bacillary angiomatosis and bacillary peliosis. Conflicting reports describe the pathogenesis of B. henselae in the cat. In this study, we characterized a strain of B. henselae termed LSU16. This strain was isolated on rabbit blood agar from a naturally infected 10-month-old female cat during a recurrent episode of bacteremia. The bacterial species was confirmed by PCR-restriction fragment length polymorphism analysis. Nine cats were infected intradermally with 5 × 107 CFU of LSU16, and clinical signs, antibody responses, and bacteremia were monitored. All nine cats developed raised, erythematous areas at the site of inoculation within 72 h postinoculation; the swelling peaked at 14 days postinfection and was not palpable by 28 days postinfection. Fever developed in all nine cats between 6 and 16 days postinfection and lasted for 1 to 8 days. Between 6 and 16 days postinfection, all nine cats experienced lethargy which persisted 5 to 18 days. Seven of nine cats were bacteremic by day 7, and all nine cats had become bacteremic by 14 days postinfection. Bacteremia peaked at 14 to 28 days postinfection in all cats. In six of the nine infected cats, bacterial numbers reached nondetectable levels during the 7th week postinfection; however, a single animal maintained bacteremia to 18 weeks postinfection. All nine cats developed strong antibody responses toB. henselae, as determined by Western blot analysis and enzyme-linked immunosorbent assay. Subsequently, three naive cats were injected intradermally with blood from cats infected with LSU16 from a pure culture, and five naive cats were injected with feces from fleas which had been feeding on cats infected with a pure culture of LSU16. These cats developed signs similar to those described in the previous experiment and were euthanized at 5 weeks postinfection. We conclude that B. henselae LSU16 is a virulent strain of B. henselae in cats and propose that the virulence of B. henselae in cats is strain dependent.
机译：亨通巴尔通体是人类猫抓伤病以及几种严重感染后遗症的病原体，包括细菌性血管瘤病和细菌性乳糜泻。相互矛盾的报道描述了 B的发病机理。 henselae 在猫中。在这项研究中，我们表征了 B菌株。 henselae 称为LSU16。在反复发生菌血症期间，从一只自然感染的10个月大的母猫的兔血琼脂上分离出该菌株。通过PCR-限制性片段长度多态性分析确认了细菌种类。 9只猫皮内感染了5×10 7 CSU LSU16，并对其临床体征，抗体反应和菌血症进行了监测。接种后72小时内，所有9只猫在接种部位均出现凸起的红斑区域。肿胀在感染后第14天达到峰值，并且在感染后第28天无法触及。感染后6至16天，所有9只猫都出现了发烧，持续1至8天。感染后6至16天，所有9只猫都出现嗜睡，持续5至18天。到第7天时，九只猫中有七只具有细菌性，到感染后14天，所有九只猫都具有细菌性。在所有猫中，细菌血症在感染后14至28天达到峰值。在感染的9只猫中，有6只在感染后第7周达到了无法检测到的细菌水平。但是，一只动物在感染后18周内仍保持菌血症。所有九只猫都对 B产生强烈的抗体反应。蛋白质印迹分析和酶联免疫吸附法测定随后，向三只幼稚猫皮内注射来自纯培养物感染LSU16的猫的血液，五只幼稚猫注射来自跳蚤的粪便，这些粪便以感染了纯LSU16培养物的猫为食。这些猫出现与先前实验中所述相似的体征，并在感染后5周安乐死。我们得出结论， B。 henselae LSU16是 B的强毒株。 henselae 在猫中的表达，并提出 B的毒力。猫中的henselae 是应变依赖的。

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