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首页> 外文期刊>Infection and immunity >Enterotoxicity and Cytotoxicity of Vibrio parahaemolyticus Thermostable Direct Hemolysin in In Vitro Systems
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Enterotoxicity and Cytotoxicity of Vibrio parahaemolyticus Thermostable Direct Hemolysin in In Vitro Systems

机译：副溶血性弧菌热稳定的直接溶血素在体外系统中的肠毒性和细胞毒性

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摘要

Vibrio parahaemolyticus is a marine bacterium known to be a common cause of seafood gastroenteritis worldwide. The thermostable direct hemolysin (TDH) has been proposed to be a major virulence factor of V. parahaemolyticus. TDH causes intestinal fluid secretion as well as cytotoxicity in a variety of cell types. In this study, we investigated the interplay between the hemolysin's enterotoxic and cytotoxic effects by using both human and rat cell monolayers. As revealed by microspectrofluorimetry, the toxin causes a dose-dependent increase in intracellular free calcium in both Caco-2 and IEC-6 cells. This effect was reversible only when low toxin concentrations were tested. The TDH-activated ion influx pathway is not selective for calcium but admits ions such sodium and manganese as well. Furthermore, in the same range of concentration, the hemolysin triggers a calcium-dependent chloride secretion. At high concentrations, TDH induces a dose-dependent but calcium-independent cell death as assessed by functional, biochemical, and morphological assays.
机译：副溶血性弧菌是一种海洋细菌，已知是全球海鲜胃肠炎的常见病因。已经提出热稳定的直接溶血素（TDH）是副溶血性弧菌的主要毒力因子。 TDH在多种细胞类型中引起肠液分泌以及细胞毒性。在这项研究中，我们通过使用人和大鼠细胞单层研究了溶血素的肠毒性和细胞毒性作用之间的相互作用。如显微荧光法所揭示，该毒素在Caco-2和IEC-6细胞中引起细胞内游离钙的剂量依赖性增加。仅当测试低毒素浓度时，此作用才可逆。 TDH激活的离子流入途径对钙不是选择性的，但也可以吸收钠和锰等离子。此外，在相同的浓度范围内，溶血素触发钙依赖性氯的分泌。通过功能，生化和形态学分析评估，在高浓度下，TDH诱导剂量依赖性但不依赖钙的细胞死亡。

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